| Cyclic AMP-independent ATF family members interact with NF-kappa B and function in the activation of the E-selectin promoter in response to cytokines. | |
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MedLine Citation:
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PMID: 7692236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that NF-kappa B and a complex we referred to as NF-ELAM1 play a central role in cytokine-induced expression of the E-selectin gene. In this study we identify cyclic AMP (cAMP)-independent members of the ATF family binding specifically to the NF-ELAM1 promoter element. The NF-ELAM1 element (TGACATCA) differs by a single nucleotide substitution from the cAMP-responsive element consensus sequence. We demonstrate that this sequence operates in a cAMP-independent manner to induce transcription and thus define it as a non-cAMP-responsive element (NCRE). We show that ATFa is a component of the NF-ELAM1 complex and its overexpression activates the E-selectin promoter. In addition, ATFa, ATF2, and ATF3 interact directly with NF-kappa B in vitro, linking two unrelated families of transcription factors in a novel protein-protein interaction. Furthermore, we demonstrate that the ability of overexpressed NF-kappa B to transactivate the E-selectin promoter in vivo is dependent on the NF-ELAM1 complex. Our results suggest that a direct interaction between ATFs and NF-kappa B is, at least in part, the mechanism by which these factors specifically regulate E-selectin promoter activity. |
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Authors:
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W Kaszubska; R Hooft van Huijsduijnen; P Ghersa; A M DeRaemy-Schenk; B P Chen; T Hai; J F DeLamarter; J Whelan |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular and cellular biology Volume: 13 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-11-18 Completed Date: 1993-11-18 Revised Date: 2010-02-04 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7180-90 Citation Subset: IM |
Affiliation:
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Glaxo Institute for Molecular Biology, Geneva, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Activating Transcription Factors Base Sequence Binding Sites Blood Proteins / metabolism* Cell Adhesion Molecules / biosynthesis*, genetics* Cloning, Molecular Cyclic AMP / pharmacology* Cytokines / pharmacology* DNA, Complementary / biosynthesis, metabolism E-Selectin Endothelium, Vascular / metabolism Forskolin / pharmacology Gene Expression Regulation / drug effects* Gene Library Glutathione Transferase / biosynthesis, metabolism Hela Cells Humans Leucine Zippers Membrane Glycoproteins / biosynthesis Molecular Sequence Data NF-kappa B / metabolism* Neoplasm Proteins / metabolism Oligonucleotide Probes Plasmids Promoter Regions, Genetic* / drug effects Recombinant Fusion Proteins / metabolism Transcription Factors / metabolism* Umbilical Veins |
| Chemical | |
Reg. No./Substance:
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0/Activating Transcription Factors; 0/Blood Proteins; 0/Cell Adhesion Molecules; 0/Cytokines; 0/DNA, Complementary; 0/E-Selectin; 0/Membrane Glycoproteins; 0/NF-kappa B; 0/Neoplasm Proteins; 0/Oligonucleotide Probes; 0/Recombinant Fusion Proteins; 0/Transcription Factors; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC 2.5.1.18/Glutathione Transferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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