Document Detail

Cyclic AMP formation in rat bone and kidney cells is stimulated equally by parathyroid hormone-related protein (PTHrP) 1-34 and PTH 1-34.
MedLine Citation:
PMID:  8223983     Owner:  NLM     Status:  MEDLINE    
Parathyroid hormone-related protein (PTHrP) plays a major role in the pathogenesis of humoral hypercalcemia of malignancy. It interacts with the PTH receptor and has therefore a nearly identical effect on bone cells as PTH. However, PTHrP is thought to be less potent than PTH in stimulating adenylate cyclase in canine renal membranes, leading to the hypothesis of a differential efficiency in signal transduction by PTHrP with respect to bone vs kidney. In a homologous model with intact osteoblast-like cells (UMR 106) and primary kidney cells, both from the rat, we have tested N-terminal peptide fragments, based on the rat amino acid sequence 1-34, of PTH and PTHrP. Compared with PTHrP(1-34), rat PTH(1-34) had a similar relative potency in bone cells (85%) and in kidney cells (140%) in its ability to stimulate adenylate cyclase. Human PTH(1-34) was 5.6- to 6.5-fold less potent than rat PTH(1-34) in both cell types. In human osteoblast-like cells (SaOS-2), rat and human PTH were essentially equally potent compared to PTHrP(1-34) (identical sequence in rat and human) in stimulating cAMP accumulation. In conclusion, our study revealed the equipotency of rat PTH(1-34) and PTHrP(1-34) in stimulating intracellular cAMP formation in a homologous system of rat bone and kidney cells. There seemed to be no unique signal transduction mechanism of PTHrP to the adenylate cyclase in rat kidney cells compared with bone cells.
E Blind; F Raue; V Knappe; J Schroth; R Ziegler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental and clinical endocrinology     Volume:  101     ISSN:  0232-7384     ISO Abbreviation:  Exp. Clin. Endocrinol.     Publication Date:  1993  
Date Detail:
Created Date:  1993-12-20     Completed Date:  1993-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302802     Medline TA:  Exp Clin Endocrinol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  150-5     Citation Subset:  IM    
Department of Internal Medicine I, Endocrinology and Metabolism, University of Heidelberg, Germany.
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MeSH Terms
Adenylate Cyclase / metabolism
Antihypertensive Agents / pharmacology*
Bone and Bones / cytology,  metabolism*
Cell Line
Cyclic AMP / biosynthesis*
Kidney / cytology,  metabolism*
Osteoblasts / drug effects,  metabolism
Parathyroid Hormone / pharmacology*
Parathyroid Hormone-Related Protein*
Peptide Fragments / pharmacology*
Proteins / pharmacology*
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Antihypertensive Agents; 0/Parathyroid Hormone; 0/Parathyroid Hormone-Related Protein; 0/Peptide Fragments; 0/Proteins; 0/Recombinant Proteins; 112540-82-6/parathyroid hormone-related protein (1-34); 52232-67-4/Teriparatide; 60-92-4/Cyclic AMP; EC Cyclase

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