Document Detail

Cyclic AMP and cyclic GMP accumulation by isolated hamster granulosa cells stimulated by LH and FSH.
MedLine Citation:
PMID:  211776     Owner:  NLM     Status:  MEDLINE    
Cyclic AMP and cyclic GMP accumulation in hamster granulosa cells as a function of gonadotrophin dose (LH or FSH) and time (0-30 min) was determined. The pattern of acute cyclic AMP accumulation was similar in LH and FSH stimulated granulosa cells, except that the cells were more sensitive to FSH than LH. There was a positive dose response relationship of cyclic AMP accumulation in LH and FSH stimulated cells. LH appeared to partially inhibit FSH stimulated cyclic AMP synthesis. Cyclic GMP accumulation was distinctly different in LH and FSH stimulated cells. An inverse dose response relationship of cyclic GMP to dose LH was observed, with only the lowest dose of LH (0.005 IU/ml) stimulating cyclic GMP synthesis. FSH at 0.005 IU/ml did not stimulate cyclic GMP synthesis, but at higher doses generated cyclic GMP in a positive dose-related manner. The results suggest that specificity of hormone action in granulosa cells may be governed in part by differential on cyclic AMP and cyclic GMP in these cells.
A Makris; K J Ryan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Acta endocrinologica     Volume:  89     ISSN:  0001-5598     ISO Abbreviation:  Acta Endocrinol.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-11-18     Completed Date:  1978-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370312     Medline TA:  Acta Endocrinol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  173-81     Citation Subset:  IM    
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MeSH Terms
Cell Separation
Cyclic AMP / metabolism*
Cyclic GMP / metabolism*
Dose-Response Relationship, Drug
Follicle Stimulating Hormone / pharmacology*
Granulosa Cells / metabolism*
Luteinizing Hormone / pharmacology*
Stimulation, Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP; 7665-99-8/Cyclic GMP; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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