Document Detail


Cyclic 2,3-diphosphoglycerate as a component of a new branch in gluconeogenesis in Methanobacterium thermoautotrophicum delta H.
MedLine Citation:
PMID:  8320225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A unique compound, cyclic 2,3-diphosphoglycerate (cDPG), is the major soluble carbon and phosphorus solute in Methanobacterium thermoautotrophicum delta H under optimal conditions of cell growth. It is a component of an unusual branch in gluconeogenesis in these bacteria. [U-13C]acetate pulse-[12C]acetate chase methodology was used to observe the relationship between cDPG and other metabolites (2-phosphoglycerate and 2,3-diphosphoglycerate [2-PG and 2,3-DPG, respectively]) of this branch. It was demonstrated that cells could grow exponentially under conditions in which 2-PG and 2,3-DPG, rather than cDPG, were the major solutes. While the total concentration of these three phosphorylated molecules was maintained, rapid interconversion of 13C label among them was observed. Label flow from 2-PG to 2,3-DPG to cDPG to polymer is the usual direction in this pathway in exponentially growing cells, while the reverse reactions sometimes predominate in the stationary phase. Evidence of the presence of a polymeric compound in this pathway was provided by 13C nuclear magnetic resonance (one-dimensional and two-dimensional INADEQUATE) studies of solubilized cell debris.
Authors:
A Gorkovenko; M F Roberts
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  175     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-07-30     Completed Date:  1993-07-30     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4087-95     Citation Subset:  IM    
Affiliation:
Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02167.
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MeSH Terms
Descriptor/Qualifier:
2,3-Diphosphoglycerate
Cell Extracts / chemistry
Diphosphoglyceric Acids / analysis*,  metabolism
Gluconeogenesis*
Glyceric Acids / metabolism
Magnetic Resonance Spectroscopy
Methanobacterium / chemistry*
Models, Biological
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Diphosphoglyceric Acids; 0/Glyceric Acids; 138-81-8/2,3-Diphosphoglycerate; 2553-59-5/2-phosphoglycerate; 88280-54-0/cyclic-2,3-diphosphoglycerate
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