Document Detail

Cybrid formation in mouse L cells: the influence of cytoplast-to-cell ratio.
MedLine Citation:
PMID:  605388     Owner:  NLM     Status:  MEDLINE    
The frequency of cybrid colony formation was measured in fusions between enucleated chloramphenicol (CAP)-resistant mouse cells and CAP-sensitive mouse cells in varying ratios. By labeling the CAP-resistant cytoplasts with polystyrene beads and then performing the same fusions with CAP-sensitive cells, the frequency of cybrid fusions could be measured. Comparison of the frequency of viable cybrids (cybrid colonies) with the frequency of cybrid fusions showed that, with increasing fusion ratios of cytoplasts to cells, the proportion of cells fused to cytoplasts increased. Further, the viability of cybrid fusions increased from about 1 in 500 to nearly 1 in 60 over the range of cytoplast-to-cell ratios studied.
C L Bunn; J M Eisenstadt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell genetics     Volume:  3     ISSN:  0098-0366     ISO Abbreviation:  Somatic Cell Genet.     Publication Date:  1977 May 
Date Detail:
Created Date:  1978-04-17     Completed Date:  1978-04-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506054     Medline TA:  Somatic Cell Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  335-41     Citation Subset:  IM    
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MeSH Terms
Cell Fusion
Cell Survival
Chloramphenicol / pharmacology
Cytoplasm / physiology*
Drug Resistance
Hybrid Cells / physiology*
L Cells (Cell Line) / physiology*
Reg. No./Substance:

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