| Cyanide-induced injury to the isolated perfused rat liver. | |
| | |
MedLine Citation:
|
PMID: 3237595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In order to study the events that follow cyanide-induced inhibition of oxidative metabolism and produce cellular injury, isolated, haemoglobin-free perfused rat livers from fasted rats were exposed to KCN (100 mg/l). KCN reduced the oxygen consumption of the livers by about 80%. Hepatotoxicity was evident by a marked release of enzymes (LDH, SDH) and of glutathione (mainly GSSG) into the perfusate, by a depletion of hepatic glutathione and by an accumulation of calcium in the liver. Cyanide-induced hepatotoxicity could be prevented completely by feeding the rats before preparing the liver as well as by addition of fructose to the perfusate of fasted livers. Both treatments resulted in an increased energy supply from anaerobic glycolysis as evidenced by a large release of lactate + pyruvate into the perfusate. The toxic actions of cyanide were markedly attenuated by deferrioxamine as well as by allopurinol. These antitoxic actions occurred without changes in anaerobic glycolysis. Omission of calcium from the perfusate, however, did not influence cyanide toxicity. Thus, energy supply from anaerobic glycolysis seems to be sufficient for the basic functions of the liver to occur, when oxidative metabolism is inhibited by cyanide. The effects of deferrioxamine and allopurinol indicate the involvement of radical intermediates and/or Fe2+ in cyanide-induced cellular toxicity. An influx of calcium from the extracellular to the intracellular space is not involved in cyanide-induced hepatocellular injury. |
| | |
Authors:
|
M Younes; O Strubelt |
Related Documents
:
|
6672795 - In vivo release of dopamine during perfusion of neurotensin in substantia nigra of the ... 209115 - Catabolism of the apoprotein of low density lipoproteins by the isolated perfused rat l... 11397395 - First-pass metabolism of 1,3-butadiene in once-through perfused livers of rats and mice. 6698205 - High resolution proton nmr studies of perfused rat hearts. 2598325 - Synthesis and biological activities of optical isomers of 2-(4-diphenylmethyl-1-piperaz... 6029605 - The stereochemistry of the hydrogen elimination in the biological conversion of cholest... |
Publication Detail:
|
Type: Comparative Study; In Vitro; Journal Article |
Journal Detail:
|
Title: Pharmacology & toxicology Volume: 63 ISSN: 0901-9928 ISO Abbreviation: Pharmacol. Toxicol. Publication Date: 1988 Nov |
Date Detail:
|
Created Date: 1989-05-02 Completed Date: 1989-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8702180 Medline TA: Pharmacol Toxicol Country: DENMARK |
Other Details:
|
Languages: eng Pagination: 382-5 Citation Subset: IM |
Affiliation:
|
Institute of Toxicology, Medical University of Lübeck, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Calcium / metabolism Cyanides / toxicity* Glutathione / metabolism Lactates / metabolism Liver / drug effects*, metabolism Oxygen Consumption Perfusion Potassium Cyanide / toxicity* Pyruvates / metabolism Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Cyanides; 0/Lactates; 0/Pyruvates; 151-50-8/Potassium Cyanide; 70-18-8/Glutathione; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gastrointestinal absorption and urinary excretion of aluminium in patients with predialysis chronic ...
Next Document: Actions of terodiline, its isomers and main metabolite on isolated detrusor muscle from rabbit and m...