Document Detail


Cyanide-induced alteration of the adenylate energy pool in a rat neurosecretory cell line.
MedLine Citation:
PMID:  2061557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultures of a rat PC12 pheochromocytoma neurosecretory cell line were used to determine the responsiveness of oxidative energy status of isolated neuronal cells to cyanide exposure. Intracellular levels of ATP and its immediate metabolites, ADP and AMP, were measured in monolayer cultures of PC12 cells incubated for 0-30 min with KCN (10 mM). Over the period 2.5-30 min. cyanide treatment decreased ATP levels by 32-51% but ADP and AMP levels were not altered significantly. Additionally, ATP/ADP and ATP/AMP ratios were significantly reduced in KCN-intoxicated cells. These alterations in energy status may explain the prompt ablation of ion homeostasis reported previously in this model upon exposure to KCN. The energy-depleting actions of cyanide were not modified by pretreatment of cells with diltiazem, a calcium channel antagonist demonstrated to possess cytoprotective activity against histotoxic hypoxia induced by cyanide. Since PC12 cells rapidly respond to cyanide, with predictable depletions of the cell adenylate energy pool, this cell line can serve as a suitable in vitro model for studies of neurotoxicity involving ischemic/hypoxic conditions.
Authors:
E U Maduh; J L Borowitz; G E Isom
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied toxicology : JAT     Volume:  11     ISSN:  0260-437X     ISO Abbreviation:  J Appl Toxicol     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-08-07     Completed Date:  1991-08-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8109495     Medline TA:  J Appl Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-101     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907.
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MeSH Terms
Descriptor/Qualifier:
Adenine Nucleotides / metabolism*
Animals
Chromatography, High Pressure Liquid
Diltiazem / pharmacology
Energy Metabolism / drug effects*
Neurons / drug effects*,  metabolism
Neurosecretory Systems / drug effects*,  metabolism
Potassium Cyanide / antagonists & inhibitors,  toxicity*
Rats
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
5T32ES07039/ES/NIEHS NIH HHS; ES04140/ES/NIEHS NIH HHS; S07RR05586/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Adenine Nucleotides; 151-50-8/Potassium Cyanide; 42399-41-7/Diltiazem

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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