Document Detail

Cyanide-induced alteration of the adenylate energy pool in a rat neurosecretory cell line.
MedLine Citation:
PMID:  2061557     Owner:  NLM     Status:  MEDLINE    
Cultures of a rat PC12 pheochromocytoma neurosecretory cell line were used to determine the responsiveness of oxidative energy status of isolated neuronal cells to cyanide exposure. Intracellular levels of ATP and its immediate metabolites, ADP and AMP, were measured in monolayer cultures of PC12 cells incubated for 0-30 min with KCN (10 mM). Over the period 2.5-30 min. cyanide treatment decreased ATP levels by 32-51% but ADP and AMP levels were not altered significantly. Additionally, ATP/ADP and ATP/AMP ratios were significantly reduced in KCN-intoxicated cells. These alterations in energy status may explain the prompt ablation of ion homeostasis reported previously in this model upon exposure to KCN. The energy-depleting actions of cyanide were not modified by pretreatment of cells with diltiazem, a calcium channel antagonist demonstrated to possess cytoprotective activity against histotoxic hypoxia induced by cyanide. Since PC12 cells rapidly respond to cyanide, with predictable depletions of the cell adenylate energy pool, this cell line can serve as a suitable in vitro model for studies of neurotoxicity involving ischemic/hypoxic conditions.
E U Maduh; J L Borowitz; G E Isom
Related Documents :
337307 - Specific neural and adrenal medullary antigens detected by antisera to clonal pc12 pheo...
16766477 - Degradation of organophosphorus neurotoxicity in sy5y neuroblastoma cells by organophos...
748757 - Phenotypic modulation in mouse neuroblastoma cells: the case of acetylcholinesterase.
16777077 - Characterization of human dopamine responsive protein drg-1 that binds to p75ntr-associ...
22939887 - Efficacy and mechanism of action of proellex, an antiprogestin in aromatase overexpress...
21454517 - Rap1-gtp-interacting adaptor molecule (riam) protein controls invasion and growth of me...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied toxicology : JAT     Volume:  11     ISSN:  0260-437X     ISO Abbreviation:  J Appl Toxicol     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-08-07     Completed Date:  1991-08-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8109495     Medline TA:  J Appl Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-101     Citation Subset:  IM    
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, IN 47907.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenine Nucleotides / metabolism*
Chromatography, High Pressure Liquid
Diltiazem / pharmacology
Energy Metabolism / drug effects*
Neurons / drug effects*,  metabolism
Neurosecretory Systems / drug effects*,  metabolism
Potassium Cyanide / antagonists & inhibitors,  toxicity*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Adenine Nucleotides; 151-50-8/Potassium Cyanide; 42399-41-7/Diltiazem

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Gene mutation assay of acrolein in the CHO/HGPRT test system.
Next Document:  Test and calibration processes for microcalorimeters, with special reference to heat conduction inst...