Document Detail


Novel function of B cell-activating factor in the induction of IL-10-producing regulatory B cells.
MedLine Citation:
PMID:  20208006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although B cells have been shown to possess a regulatory function, microenvironmental factors or cytokines involved in the induction of regulatory B cells remain largely uncharacterized. B cell-activating factor (BAFF), a member of TNF family cytokines, is a key regulator for B cell maturation and function. In this study, we detected significantly increased numbers of IL-10-producing B cells in BAFF-treated B cell cultures, an effect specifically abrogated by neutralization of BAFF with TACI-Fc. BAFF-induced IL-10-producing B cells showed a distinct CD1d(hi)CD5(+) phenotype, which were mainly derived from marginal zone B cells. Moreover, BAFF activated transcription factor AP-1 for binding to IL-10 promoter. Notably, BAFF treatment in vivo increased the number of IL-10-producing B cells in marginal zone regions. Furthermore, BAFF-induced IL-10-producing B cells possess a regulatory function both in vitro and in vivo. Taken together, our findings identify a novel function of BAFF in the induction of IL-10-producing regulatory B cells.
Authors:
Min Yang; Lingyun Sun; Shengjun Wang; King-Hung Ko; Huaxi Xu; Bo-Jian Zheng; Xuetao Cao; Liwei Lu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-05
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  184     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3321-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology and Center of Infection and Immunology, The University of Hong Kong, Hong Kong.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Cell Activating Factor / immunology*,  metabolism
B-Lymphocyte Subsets / immunology*,  metabolism
B-Lymphocytes / immunology*,  metabolism
Cell Differentiation / immunology*
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Gene Expression
Gene Expression Regulation / immunology
Interleukin-10 / biosynthesis*,  immunology
Mice
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factor AP-1 / biosynthesis,  immunology
Chemical
Reg. No./Substance:
0/B-Cell Activating Factor; 0/Tnfsf13b protein, mouse; 0/Transcription Factor AP-1; 130068-27-8/Interleukin-10

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