Document Detail


Cutting edge: intravascular staining redefines lung CD8 T cell responses.
MedLine Citation:
PMID:  22896631     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease.
Authors:
Kristin G Anderson; Heungsup Sung; Cara N Skon; Leo Lefrancois; Angela Deisinger; Vaiva Vezys; David Masopust
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-08-15
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  189     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-07     Completed Date:  2012-11-26     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2702-6     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / administration & dosage,  diagnostic use
Antigens, CD8 / immunology
CD8-Positive T-Lymphocytes / immunology*,  pathology*,  transplantation
Capillaries / immunology*,  pathology*,  virology
Cell Movement / genetics,  immunology
Lung / blood supply*,  immunology*,  metabolism
Lymphocytic Choriomeningitis / genetics,  immunology,  pathology
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Pertussis Toxin / administration & dosage
Pneumonia, Viral / genetics,  immunology,  pathology
Spleen / immunology,  pathology,  transplantation
Staining and Labeling / methods
Grant Support
ID/Acronym/Agency:
R01 AI084913/AI/NIAID NIH HHS; R01AI084913/AI/NIAID NIH HHS; T32 AI007313/AI/NIAID NIH HHS; T32-AI07313/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD8; 0/CD8 antigen, alpha chain; 0/Cd8b1 protein, mouse; EC 2.4.2.31/Pertussis Toxin
Comments/Corrections

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