| Cutting edge: intravascular staining redefines lung CD8 T cell responses. | |
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MedLine Citation:
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PMID: 22896631 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease. |
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Authors:
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Kristin G Anderson; Heungsup Sung; Cara N Skon; Leo Lefrancois; Angela Deisinger; Vaiva Vezys; David Masopust |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-15 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 189 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-07 Completed Date: 2012-11-26 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 2702-6 Citation Subset: AIM; IM |
Affiliation:
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Department of Microbiology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / administration & dosage, diagnostic use Antigens, CD8 / immunology CD8-Positive T-Lymphocytes / immunology*, pathology*, transplantation Capillaries / immunology*, pathology*, virology Cell Movement / genetics, immunology Lung / blood supply*, immunology*, metabolism Lymphocytic Choriomeningitis / genetics, immunology, pathology Mice Mice, Inbred C57BL Mice, Mutant Strains Pertussis Toxin / administration & dosage Pneumonia, Viral / genetics, immunology, pathology Spleen / immunology, pathology, transplantation Staining and Labeling / methods |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI084913/AI/NIAID NIH HHS; R01AI084913/AI/NIAID NIH HHS; T32-AI07313/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigens, CD8; 0/CD8 antigen, alpha chain; 0/Cd8b1 protein, mouse; EC 2.4.2.31/Pertussis Toxin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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