| Cutting edge: CTNNBL1 is dispensable for Ig class switch recombination. | |
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MedLine Citation:
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PMID: 20585033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ig class switch recombination (CSR) and somatic hypermutation require activation-induced cytidine deaminase (AID). The search for AID-interaction factors has been a major research effort in the field, as the mechanism of preferential targeting of AID to Ig loci remains elusive. CTNNBL1 is one of the few identified AID-interacting factors and has been shown to affect AID-mediated mutation and gene conversion in chicken DT40 cells. CTNNBL1 was also implicated in mammalian CSR by the fact that an AID mutant that fails to interact with CTNNBL1 also fails to support CSR in AID-deficient mouse B cells. To directly assess the role of CTNNBL1 in CSR, we disrupted the CTNNBL1 gene on both alleles in mouse CH12F3 cells by gene targeting. We found normal levels of CSR in CTNNBL1-deficient cells, indicating that CTNNBL1 is dispensable for CSR. |
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Authors:
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Li Han; Shahnaz Masani; Kefei Yu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-06-28 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-09-08 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 1379-81 Citation Subset: AIM; IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis Regulatory Proteins / deficiency, genetics, physiology* Cell Line Cell Line, Tumor Cytidine Deaminase / deficiency, genetics, physiology Gene Targeting Immunoglobulin A / genetics, metabolism Immunoglobulin Class Switching / genetics* Mice Mice, Knockout Nuclear Proteins / deficiency, genetics, physiology* Recombination, Genetic* / immunology beta Catenin |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI081817/AI/NIAID NIH HHS; R01 AI081817-01A1/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Regulatory Proteins; 0/CTNNBL1 protein, mouse; 0/Immunoglobulin A; 0/Nuclear Proteins; 0/beta Catenin; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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