| Cutting edge: The adapters EAT-2A and -2B are positive regulators of CD244- and CD84-dependent NK cell functions in the C57BL/6 mouse. | |
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MedLine Citation:
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PMID: 20962259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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EWS/FLI1-activated transcript 2 (EAT-2)A and EAT-2B are single SH2-domain proteins, which bind to phosphorylated tyrosines of signaling lymphocyte activation molecule family receptors in murine NK cells. While EAT-2 is a positive regulator in human cells, a negative regulatory role was attributed to the adapter in NK cells derived from EAT-2A-deficient 129Sv mice. To evaluate whether the genetic background or the presence of a selection marker in the mutant mice could influence the regulatory mode of these adapters, we generated EAT-2A-, EAT-2B-, and EAT-2A/B-deficient mice using C57BL/6 embryonic stem cells. We found that NK cells from EAT-2A- and EAT-2A/B-deficient mice were unable to kill tumor cells in a CD244- or CD84-dependent manner. Furthermore, EAT-2A/B positively regulate phosphorylation of Vav-1, which is known to be implicated in NK cell killing. Thus, as in humans, the EAT-2 adapters act as positive regulators of signaling lymphocyte activation molecule family receptor-specific NK cell functions in C57BL/6 mice. |
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Authors:
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Ninghai Wang; Silvia Calpe; Jill Westcott; Wilson Castro; Chunyan Ma; Pablo Engel; John D Schatzle; Cox Terhorst |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-20 |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-04 Completed Date: 2010-12-02 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 5683-7 Citation Subset: AIM; IM |
Affiliation:
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Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA. nwang@bidmc.harvard.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing Animals Antigens, CD / immunology* Blotting, Western Cell Separation Cytotoxicity, Immunologic Flow Cytometry Immunoprecipitation Killer Cells, Natural / immunology* Lymphocyte Activation / immunology* Mice Mice, Inbred C57BL Mice, Mutant Strains Receptors, Immunologic / immunology* Transcription Factors / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AI067803/AI/NIAID NIH HHS; P01 AI-065687/AI/NIAID NIH HHS; P01 AI065687-01A1/AI/NIAID NIH HHS; R01 AI067803-01A2/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Antigens, CD; 0/Cd244 protein, mouse; 0/Cd84 protein, mouse; 0/Receptors, Immunologic; 0/Sh2d1b1 protein, mouse; 0/Transcription Factors |
| Comments/Corrections | |
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