| Cutin deficiency in the tomato fruit cuticle consistently affects resistance to microbial infection and biomechanical properties, but not transpirational water loss. | |
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MedLine Citation:
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PMID: 19594708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plant cuticles are broadly composed of two major components: polymeric cutin and a mixture of waxes, which infiltrate the cutin matrix and also accumulate on the surface, forming an epicuticular layer. Although cuticles are thought to play a number of important physiological roles, with the most important being to restrict water loss from aerial plant organs, the relative contributions of cutin and waxes to cuticle function are still not well understood. Tomato (Solanum lycopersicum) fruits provide an attractive experimental system to address this question as, unlike other model plants such as Arabidopsis, they have a relatively thick astomatous cuticle, providing a poreless uniform material that is easy to isolate and handle. We identified three tomato mutants, cutin deficient 1 (cd1), cd2 and cd3, the fruit cuticles of which have a dramatic (95-98%) reduction in cutin content and substantially altered, but distinctly different, architectures. This cutin deficiency resulted in an increase in cuticle surface stiffness, and in the proportions of both hydrophilic and multiply bonded polymeric constituents. Furthermore, our data suggested that there is no correlation between the amount of cutin and the permeability of the cuticle to water, but that cutin plays an important role in protecting tissues from microbial infection. The three cd mutations were mapped to different loci, and the cloning of CD2 revealed it to encode a homeodomain protein, which we propose acts as a key regulator of cutin biosynthesis in tomato fruit. |
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Authors:
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Tal Isaacson; Dylan K Kosma; Antonio J Matas; Gregory J Buda; Yonghua He; Bingwu Yu; Arika Pravitasari; James D Batteas; Ruth E Stark; Matthew A Jenks; Jocelyn K C Rose |
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Publication Detail:
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Type: Journal Article Date: 2009-07-06 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 60 ISSN: 1365-313X ISO Abbreviation: Plant J. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-14 Completed Date: 2009-12-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 363-77 Citation Subset: IM |
Affiliation:
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Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/GQ222185 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Mapping Cloning, Molecular DNA, Plant / genetics Fruit / genetics, physiology* Gene Expression Regulation, Plant Homeodomain Proteins / genetics, metabolism Lycopersicon esculentum / genetics*, physiology Magnetic Resonance Imaging Membrane Lipids / metabolism* Microscopy, Atomic Force Microscopy, Electron, Scanning Microscopy, Electron, Transmission Molecular Sequence Data Mutation Phenotype Plant Diseases / genetics* Plant Proteins / genetics, metabolism Plant Transpiration / genetics, physiology* Water / metabolism* Waxes / chemistry |
| Chemical | |
Reg. No./Substance:
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0/DNA, Plant; 0/Homeodomain Proteins; 0/Membrane Lipids; 0/Plant Proteins; 0/Waxes; 54990-88-4/cutin; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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