Document Detail

Cutin deficiency in the tomato fruit cuticle consistently affects resistance to microbial infection and biomechanical properties, but not transpirational water loss.
MedLine Citation:
PMID:  19594708     Owner:  NLM     Status:  MEDLINE    
Plant cuticles are broadly composed of two major components: polymeric cutin and a mixture of waxes, which infiltrate the cutin matrix and also accumulate on the surface, forming an epicuticular layer. Although cuticles are thought to play a number of important physiological roles, with the most important being to restrict water loss from aerial plant organs, the relative contributions of cutin and waxes to cuticle function are still not well understood. Tomato (Solanum lycopersicum) fruits provide an attractive experimental system to address this question as, unlike other model plants such as Arabidopsis, they have a relatively thick astomatous cuticle, providing a poreless uniform material that is easy to isolate and handle. We identified three tomato mutants, cutin deficient 1 (cd1), cd2 and cd3, the fruit cuticles of which have a dramatic (95-98%) reduction in cutin content and substantially altered, but distinctly different, architectures. This cutin deficiency resulted in an increase in cuticle surface stiffness, and in the proportions of both hydrophilic and multiply bonded polymeric constituents. Furthermore, our data suggested that there is no correlation between the amount of cutin and the permeability of the cuticle to water, but that cutin plays an important role in protecting tissues from microbial infection. The three cd mutations were mapped to different loci, and the cloning of CD2 revealed it to encode a homeodomain protein, which we propose acts as a key regulator of cutin biosynthesis in tomato fruit.
Tal Isaacson; Dylan K Kosma; Antonio J Matas; Gregory J Buda; Yonghua He; Bingwu Yu; Arika Pravitasari; James D Batteas; Ruth E Stark; Matthew A Jenks; Jocelyn K C Rose
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Publication Detail:
Type:  Journal Article     Date:  2009-07-06
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  60     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-14     Completed Date:  2009-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  363-77     Citation Subset:  IM    
Department of Plant Biology, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Chromosome Mapping
Cloning, Molecular
DNA, Plant / genetics
Fruit / genetics,  physiology*
Gene Expression Regulation, Plant
Homeodomain Proteins / genetics,  metabolism
Lycopersicon esculentum / genetics*,  physiology
Magnetic Resonance Imaging
Membrane Lipids / metabolism*
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular Sequence Data
Plant Diseases / genetics*
Plant Proteins / genetics,  metabolism
Plant Transpiration / genetics,  physiology*
Water / metabolism*
Waxes / chemistry
Reg. No./Substance:
0/DNA, Plant; 0/Homeodomain Proteins; 0/Membrane Lipids; 0/Plant Proteins; 0/Waxes; 54990-88-4/cutin; 7732-18-5/Water

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