| Cuticular waxes from ivy leaves (Hedera helix L.): analysis of high-molecular-weight esters. | |
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MedLine Citation:
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PMID: 17260699 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Soluble waxes were extracted from the cuticle of ivy (Hedera helix L.) leaves with dichloromethane in a yield of ca. 13%. The cuticular waxes were directly analysed by GC-MS, high-temperature GC-MS and ESI-MS/MS. The GC-MS analysis showed mostly n-alkanols (45.3%), monoacids (18.8%), triterpenes (9.7%), n-aldehydes (8.7%) and n-alkanes (7.7%). The high-temperature GC-MS and the ESI-MS/MS analyses showed the presence of ester waxes, namely alkyl alkanoates and alkyl coumarates. Alkyl alkanoates comprised esters of the hexadecanoic acid with n-alkanols ranging from C16 to C34. Alkyl coumarates included esters of coumaric acid with n-alkanols ranging from C16 to C32. The cuticular waxes were hydrolysed and the resulting organic and aqueous phases analysed by GC-MS. The hydrolysate showed a major increase in the quantities of n-alkanols, hexadecanoic acid and coumaric acid derived from the alkyl and acyl moieties from the ester waxes. A content of ester waxes of 38% was estimated based on the results from the GC-MS analysis of the non-hydrolysed and hydrolysed cuticular waxes. Alkyl alkanoates were analysed by ESI-MS/MS as [M + Li]+ adduct ions and the alkyl coumarates as [M - H]- deprotonated ions. The ESI-MS/MS analysis allowed the detection of a wider range of ester waxes than high-temperature GC-MS, and was shown to be a useful technique for the qualitative analysis of ester waxes from plant cuticles. |
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Authors:
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Sara Santos; Lukas Schreiber; José Graça |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Phytochemical analysis : PCA Volume: 18 ISSN: 0958-0344 ISO Abbreviation: Phytochem Anal Publication Date: 2007 Jan-Feb |
Date Detail:
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Created Date: 2007-01-30 Completed Date: 2007-02-13 Revised Date: 2012-12-10 |
Medline Journal Info:
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Nlm Unique ID: 9200492 Medline TA: Phytochem Anal Country: England |
Other Details:
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Languages: eng Pagination: 60-9 Citation Subset: IM |
Affiliation:
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Departamento de Engenharia Florestal, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisboa, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Esters / analysis* Gas Chromatography-Mass Spectrometry Hedera / chemistry* Hydrolysis Molecular Weight Plant Leaves / chemistry Spectrometry, Mass, Electrospray Ionization Waxes / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Waxes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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