Document Detail

Cuticle of Caenorhabditis elegans: its isolation and partial characterization.
Jump to Full Text
MedLine Citation:
PMID:  7251677     Owner:  NLM     Status:  MEDLINE    
The adult cuticle of the soil nematode, Caenorhabditis elegans, is a proteinaceous extracellular structure elaborated by the underlying layer of hypodermal cells during the final molt in the animal's life cycle. The cuticle is composed of an outer cortical layer connected by regularly arranged struts to an inner basal layer. The cuticle can be isolated largely intact and free of all cellular material by sonication and treatment with 1% sodium dodecyl sulfate (SDS). Purified cuticles exhibit a negative material in the basal cuticle layer. The cuticle layers differ in their solubility in sulfhydryl reducing agents, susceptibility to various proteolytic enzymes and amino acid composition. The struts, basal layer, and internal cortical layer are composed of collagen proteins that are extensively cross-linked by disulfide bonds. The external cortical layer appears to contain primarily noncollagen proteins that are extensively cross-linked by nonreducible covalent bonds. The collagen proteins extracted from the cuticle with a reducing agent can be separated by SDS-polyacrylamide gel electrophoresis into eight major species differing in apparent molecular weight.
G N Cox; M Kusch; R S Edgar
Related Documents :
10551857 - Molecular cloning and ultrastructural localization of the core protein of an eggshell m...
22195017 - The human nucleolar protein ftsj3 associates with nip7 and functions in pre-rrna proces...
8635597 - Amino-terminal deletions in the decorin core protein leads to the biosynthesis of prote...
12100557 - A collagen-like surface glycoprotein is a structural component of the bacillus anthraci...
12110907 - Cloning, high level expression and purification of rat betab2-crystallin.
21325267 - A highly conserved protein of unknown function in sinorhizobium meliloti affects srna r...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  90     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1981 Jul 
Date Detail:
Created Date:  1981-09-22     Completed Date:  1981-09-22     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-17     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Caenorhabditis / analysis,  ultrastructure*
Carbohydrates / analysis
Collagen / analysis*
Molecular Weight
Proteins / analysis*
Sodium Dodecyl Sulfate
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Proteins; 151-21-3/Sodium Dodecyl Sulfate; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
Download PDF

Print publication date: Day: 1 Month: 7 Year: 1981
Volume: 90 Issue: 1
First Page: 7 Last Page: 17
ID: 2111847
Publisher Id: 81239808
PubMed Id: 7251677

Cuticle of Caenorhabditis elegans: its isolation and partial characterization

Article Categories:
  • Articles

Previous Document:  Phagocytosis of gelatin-latex particles by a murine macrophage line is dependent on fibronectin and ...
Next Document:  Bead rings at the endoplasmic reticulum-Golgi complex boundary: morphological changes accompanying i...