Document Detail


Cutaneous microdialysis of uric acid level in the dermis: modification of in vitro recovery.
MedLine Citation:
PMID:  12636015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cutaneous microdialysis is a new tool for direct sampling and real-time monitoring in dermatological research. However, its use in general laboratory practice poses several problems, one of which is determination of the relative recovery of the target material. Uric acid, one of the major antioxidants in the skin, was analysed using cutaneous microdialysis in 11 healthy subjects. Two methods for in vitro recovery were adopted, one in which a standard solution of uric acid was used and another in which serum was used. Although differences between the two methods were found, it is suggested that establishing the in vitro recovery using serum might be a simple approach for microdialysis.
Authors:
Yong Suk Lee; Sung Jun Kim; Jun Kyu Oh; Jin Ho Chung; Hee Chul Eun
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta dermato-venereologica     Volume:  83     ISSN:  0001-5555     ISO Abbreviation:  Acta Derm. Venereol.     Publication Date:  2003  
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-06-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370310     Medline TA:  Acta Derm Venereol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  10-3     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Sanggye Paik Hospital, Inje University, Nowon Gu, Seoul, Korea. ori1@chollian.net
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MeSH Terms
Descriptor/Qualifier:
Adult
Dermis / chemistry*
Humans
Male
Microdialysis / methods*
Uric Acid / analysis*,  blood
Chemical
Reg. No./Substance:
69-93-2/Uric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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