| Cutaneous leishmaniasis caused by Leishmania (L.) major infection in Sindh province, Pakistan. | |
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MedLine Citation:
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PMID: 19467219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leishmaniasis is endemic in Pakistan and is wide-spread throughout the country. Polymerase chain reaction (PCR) was performed to identify the Leishmania species present in cutaneous leishmaniasis (CL) patients from new endemic areas of the central part of Sindh province, Pakistan. The PCR primers used were designed for the identification and differentiation of Leishmania (Leishmania) major and Leishmania (Leishmania) tropica species, and PCR bands at 620 and 830 bp of the parasite-specific kinetoplast DNA sequences was identified for L. (L.) major and L. (L.) tropica, respectively. Among a total of 144 DNA samples purified from the skin biopsies of clinically suspected CL patients, 108 (75%) were positive for PCR amplification. Out of the 108 cases, 105 (97.2%) were determined to be positive for L. (L.) major infection, and 3 (2.8%) were positive for L. (L.) tropica infection. It was concluded that CL caused by L. (L.) major is the main source of infection in the central part of Sindh province in Pakistan. This rapid screening technique could be used for the diagnosis of a large number of samples from skin lesions, which commonly contain other bacterial and fungal infections. |
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Authors:
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Abdul Manan Bhutto; Farooq Rahman Soomro; Javed Hassain Baloch; Jun Matsumoto; Hiroshi Uezato; Yoshihisa Hashiguchi; Ken Katakura |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-23 |
Journal Detail:
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Title: Acta tropica Volume: 111 ISSN: 1873-6254 ISO Abbreviation: Acta Trop. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-21 Completed Date: 2009-10-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370374 Medline TA: Acta Trop Country: Netherlands |
Other Details:
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Languages: eng Pagination: 295-8 Citation Subset: IM |
Affiliation:
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Department of Dermatology, Chandka Medical College, Doctors Colony, Bunglow No. 14, VIP Road, Larkana, Pakistan. bhutto manan1@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA Primers / genetics DNA, Kinetoplast / genetics DNA, Protozoan / genetics Endemic Diseases Humans Leishmania major / genetics, isolation & purification* Leishmania tropica / genetics, isolation & purification Leishmaniasis, Cutaneous / epidemiology, parasitology* Mass Screening / methods Pakistan / epidemiology Polymerase Chain Reaction / methods* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Kinetoplast; 0/DNA, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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