Document Detail


Cutaneous Leishmaniasis in Nalut District, The Libyan Arab Jamahiriya: A Clinicoepidemiological Study and Leishmania Species Identification.
MedLine Citation:
PMID:  22551502     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract ABSTRACT: Cutaneous leishmaniasis (CL), an endemic disease in the littoral zones of the Mediterranean area, the Middle East, East Africa, and especially in Libya, has not been fully documented. The present study clarifies the clinico-epidemiologic profile of CL and the molecular genotyping of the Leishmania spp. in the Nalut district, Libya. Two hundred and twenty-three CL patients were examined at the out-patient clinics of Nalut Hospital from March 2006 to February 2007. Cutaneous leishmaniasis was diagnosed by clinical, microscopic, culture, polymerase chain reaction (PCR), and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses. The disease was observed throughout the year, with the highest incidence rate between November and February. Fifty-nine percent of patients were younger than 20 yr. Nodulo-ulcerative lesions, indurated ulcer, papulo-ulcerative lesions, and subcutaneous nodular lesions were observed in 170, 25, 15, and 13 patients, respectively. Two hundred patients (89.7%) had dry type of lesions, while 23 patients (10.3%) presented a wet type. One hundred and fifty-nine (71.3%) of 223 patients were confirmed positive for CL by the presence of the amastigote form of Leishmania by stained Giemsa smear, and 170 (76.2%) were positive according to the presence of the promastigote form of Leishmania by culture in RPMI medium supplemented with 10% fetal bovine serum (FBS). On the other hand, PCR confirmed 203 (91.0%) positive cases. Genotyping of Leishmania spp. by RFLP analysis revealed that L. tropica was the most common spp. at all ages, and L. infantum was second under 20 yr of age. Cutaneous leishmaniasis is endemic in the Nalut district, Libya; PCR was the most sensitive parasite diagnostic test, and L. tropica was the most common spp.
Authors:
Usama S Belal; Ekhlas Hamed Abdel-Hafeez; Koji Naoi; Kazumi Norose
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-2
Journal Detail:
Title:  The Journal of parasitology     Volume:  -     ISSN:  1937-2345     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
a Department of Parasitology, Faculty of Medicine, Minia University.
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