Document Detail

Current trends and future prospects of biotechnological interventions through tissue culture in apple.
MedLine Citation:
PMID:  20697717     Owner:  NLM     Status:  MEDLINE    
Apple (Malus domestica Borkh.), which is a widely cultivated, important economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Apple cultivation is heavily dependent on climatic condition and is susceptible to several diseases caused by fungi, bacteria, viruses, insects, etc. Extensive research work has been carried out to standardize tissue culture protocols and utilize them in apple improvement. We review the in vitro shoot multiplication, rooting, transformation and regeneration methodologies in apple and tabulate various such protocols for easy reference. The utility and limitation of transgenesis in apple improvement have also been summarized. The concepts of marker-free plants, use of non-antibiotic resistance selectable markers, and cisgenic and intragenic approaches are highlighted. Furthermore, the limitations, current trends and future prospects of tissue culture-mediated biotechnological interventions in apple improvement are discussed.
Shammi Bhatti; Gopaljee Jha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2010-08-10
Journal Detail:
Title:  Plant cell reports     Volume:  29     ISSN:  1432-203X     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1215-25     Citation Subset:  IM    
Biotechnology Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176061, Himachal Pradesh, India.
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MeSH Terms
Agriculture / methods
Biotechnology / methods*
Crops, Agricultural / growth & development
Culture Media
Malus / genetics,  growth & development*
Plant Diseases / prevention & control
Plants, Genetically Modified / growth & development
Tissue Culture Techniques*
Reg. No./Substance:
0/Culture Media

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