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Current status of human pluripotent stem cell based in vitro toxicity tests .
MedLine Citation:
PMID:  23277040     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The present review assesses the current status of in vitro tests based on human pluripotent stem cell-derived toxicologically relevant target cells. The majority of the evaluated test systems are in the phase of test development. In particular the success rates of differentiation protocols and their reproducibility are varying depending on different culture conditions but also on the assessed marker panel and the functional evaluation of the cells. However, the amount of differentiated cells decreases in relation to their maturation status. No harmonization has been achieved yet about the required maturation status of the cellular models to be used for toxicological applications. Even with an established cellular model, the selection of appropriate readouts is challenging. Some areas of toxicity, such as developmental toxicity, suffer from insufficient knowledge on predictive biomarkers which leads to difficulties in the selection of the most appropriate endpoints. In this heterogeneous context the rapidly increasing knowledge about 'omics' technologies, might lead to an improvement of the current situation and allow the establishment of more predictive human in vitro toxicity tests.
Authors:
Martina Klaric; Johannes Winkler; Kinga Vojnits; Kesavan Meganathan; Smita Jagtap; Roberto Ensenat-Waser; Juergen Hescheler; Agapios Sachinidis; Susanne Bremer-Hoffmann
Publication Detail:
Type:  Journal Article     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Scholar edition)     Volume:  5     ISSN:  1945-0524     ISO Abbreviation:  Front Biosci (Schol Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485241     Medline TA:  Front Biosci (Schol Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  118-33     Citation Subset:  IM    
Affiliation:
Systems toxicology unit, ECVAM, European Centre for the Validation of Alternative Methods, T.P. 580, IHCP, JRC, European Commission, via E. Fermi 2749, 21027 Ispra,VA, Italy.
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