Document Detail

Current smoking of elderly men reduces antioxidants in alveolar macrophages.
MedLine Citation:
PMID:  8111579     Owner:  NLM     Status:  MEDLINE    
Alveolar macrophages were prepared from 15 healthy elderly male current smokers 60.9 +/- 9.9 yr of age (mean +/- SD). Alveolar macrophages from 11 elderly male nonsmokers 66.7 +/- 8.1 yr of age served as controls. Production of oxygen radical species was higher in alveolar macrophages from current smokers than in those from nonsmokers in the presence and absence of phorbol myristate acetate (29.8 +/- 15.5 versus 13.7 +/- 8.2 mumol/10(6) cells and 3.8 +/- 1.6 versus 2.2 +/- 1.2 mumol/10(6) cells, respectively, p < 0.01). Decreases in antioxidant activity were observed in cells from smokers versus those from nonsmokers for Cu, Zn-superoxide dismutase (114 +/- 41 versus 210 +/- 73 units/mg protein, respectively, p < 0.01), glutathione S-transferase (0.217 +/- 0.091 versus 0.368 +/- 0.017 units/mg protein, p < 0.01), and glutathione peroxidase (0.736 +/- 0.779 versus 1.590 +/- 0.879 units/mg protein, p < 0.05). Immunologic estimation showed a decrease in the levels of Cu, Zn-superoxide dismutase in cells from smokers (104.3 +/- 46.4 versus 184.1 +/- 64.4 ng enzyme/mg protein, respectively, p < 0.01). Northern blot analysis of Cu, Zn-superoxide dismutase mRNA showed no apparent difference between the two groups, suggesting not the inactivation of this enzyme but a reduction of the translational step or increased proteolysis. The oxidant and antioxidant imbalance observed in elderly male current smokers may be a factor in the pathogenesis of respiratory tissue injury caused by smoking.
T Kondo; S Tagami; A Yoshioka; M Nishimura; Y Kawakami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  149     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-03-28     Completed Date:  1994-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  178-82     Citation Subset:  AIM; IM    
Department of Pathological Biochemistry, Nagasaki University School of Medicine, Japan.
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MeSH Terms
Antioxidants / analysis*
Blotting, Northern
Bronchoalveolar Lavage Fluid
Case-Control Studies
Glutathione Peroxidase / analysis*
Glutathione Transferase / analysis*
Macrophages, Alveolar / chemistry*,  enzymology*
Matched-Pair Analysis
Nicotine / blood
Reactive Oxygen Species / analysis*
Smoking / adverse effects,  blood,  pathology*
Superoxide Dismutase / analysis*
Tetradecanoylphorbol Acetate / diagnostic use
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 16561-29-8/Tetradecanoylphorbol Acetate; 54-11-5/Nicotine; EC Peroxidase; EC Dismutase; EC Transferase

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