Document Detail


Current high performance liquid chromatographic methodology in analysis of nucleotides, nucleosides, and their bases. II.
MedLine Citation:
PMID:  6667419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This review of HPLC technology is presented in two parts, the first of which appeared in Volume 1, Number 5 of this journal. Part I presented an introduction to chromatography, sample preparation, and basic information on chromatographic theory and terminology, modes, and instrumentation. Also covered in Part I was a discussion of stationary and mobile phase parameters which can be varied to optimize separations. Part II of the review which follows covers peak identification, quantification, and selected applications of HPLC for metabolic profiling, for assays of adenosine and cyclic nucleotides in studies of disease processes and for enzyme assays. The paper concludes with a short discussion of future trends for HPLC in biomedical research and clinical chemistry.
Authors:
P R Brown
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer investigation     Volume:  1     ISSN:  0735-7907     ISO Abbreviation:  Cancer Invest.     Publication Date:  1983  
Date Detail:
Created Date:  1984-04-12     Completed Date:  1984-04-12     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  8307154     Medline TA:  Cancer Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  527-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Deaminase / analysis
Chromatography, High Pressure Liquid / instrumentation,  methods*
Nucleosides / analysis*
Nucleotides / analysis*
Nucleotides, Cyclic / analysis
Purines / analysis*
Pyrimidines / analysis*
Chemical
Reg. No./Substance:
0/Nucleosides; 0/Nucleotides; 0/Nucleotides, Cyclic; 0/Purines; 0/Pyrimidines; EC 3.5.4.4/Adenosine Deaminase

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