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Current challenges to viral load testing in the context of emerging genetic diversity of HIV-1.
MedLine Citation:
PMID:  23484497     Owner:  NLM     Status:  PubMed-not-MEDLINE    
INTRODUCTION: One of the major characteristics of HIV-1 is its extreme genetic diversity. A key factor in assessing the sensitivity of a molecular-based assay measuring HIV-1 RNA viral load (VL) in plasma is its ability to detect/quantify all (or most of) relevant HIV-1 genetic subtype/recombinant forms accurately.
AREAS COVERED: This review provides an overview of the current commercially available quantitative real-time assays (the Abbott RealTime HIV-1, Roche TaqMan HIV-1 versions 1.0 and 2.0, BioMérieux Nuclisens EasyQ HIV-1, Siemens VERSANT HIV-1 RNA 1.0 kinetic PCR, and Biocentric Generic HIV Viral Load assays). For each assay, studies from 2005 to 2010 assessing the impact of HIV-1 genetic diversity on the reliability of HIV-1 RNA quantification are described.
EXPERT OPINION: In light of HIV-1 genetic diversity, a general recommendation to favor one test over the other cannot categorically be made. Larger field evaluations of HIV-1 RNA assays should be conducted in areas where HIV-1 genetic diversity is the highest. The large-scale implementation of HIV-1 VL testing is urgently required in the developing world to change HIV infection from a likely death sentence into a manageable chronic infection, as done in Northern countries.
François Rouet; Florian Liégeois; Augustin Mouinga-Ondémé; Dramane Kania; Johannes Viljoen; Sammy Wambua; Nicole Ngo-Giang-Huong; Hervé Ménan; Martine Peeters; Eric Nerrienet
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Publication Detail:
Type:  Journal Article     Date:  2011-03-29
Journal Detail:
Title:  Expert opinion on medical diagnostics     Volume:  5     ISSN:  1753-0067     ISO Abbreviation:  Expert Opin Med Diagn     Publication Date:  2011 May 
Date Detail:
Created Date:  2013-03-14     Completed Date:  2013-04-19     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  101392201     Medline TA:  Expert Opin Med Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-202     Citation Subset:  -    
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