| Current applications and future trends of molecular diagnostics in clinical bacteriology. | |
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MedLine Citation:
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PMID: 19377839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular diagnostics of infectious diseases, in particular, nucleic-acid-based methods, are the fastest growing field in clinical laboratory diagnostics. These applications are stepwise replacing or complementing culture-based, biochemical, and immunological assays in microbiology laboratories. The first-generation nucleic acid assays were monoparametric such as conventional tests, determining only a single parameter. Improvements and new approaches in technology now open the possibility for the development of multiparameter assays using microarrays, multiplex nucleic acid amplification techniques, or mass spectrometry, while the introduction of closed-tube systems has resulted in rapid microbial diagnostics with a subsequently reduced contamination risk. Whereas the first assays were focused on the detection and identification of microbial pathogens, these new technologies paved the way for the parallel determination of multiple antibiotic resistance determinants or to perform microbial epidemiology and surveillance on a genetic level. |
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Authors:
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Jan Weile; Cornelius Knabbe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review Date: 2009-04-18 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 394 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-05 Completed Date: 2009-06-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 731-42 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine and Molecular Diagnostics, Robert-Bosch-Hospital, Auerbachstrasse 110, 70376, Stuttgart, Germany. jan.weile@ikp-stuttgart.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Infections
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diagnosis*,
microbiology* Mass Spectrometry Microfluidics Molecular Diagnostic Techniques / methods, trends* Oligonucleotide Array Sequence Analysis Point-of-Care Systems Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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