Document Detail


Curcumin inhibits adipogenesis in 3T3-L1 adipocytes and angiogenesis and obesity in C57/BL mice.
MedLine Citation:
PMID:  19297423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiogenesis is necessary for the growth of adipose tissue. Dietary polyphenols may suppress growth of adipose tissue through their antiangiogenic activity and by modulating adipocyte metabolism. We investigated the effect of curcumin, the major polyphenol in turmeric spice, on angiogenesis, adipogenesis, differentiation, apoptosis, and gene expression involved in lipid and energy metabolism in 3T3-L1 adipocyte in cell culture systems and on body weight gain and adiposity in mice fed a high-fat diet (22%) supplemented with 500 mg curcumin/kg diet for 12 wk. Curcumin (5-20 micromol/L) suppressed 3T3-L1 differentiation, caused apoptosis, and inhibited adipokine-induced angiogenesis of human umbilical vein endothelial cells. Supplementing the high-fat diet of mice with curcumin did not affect food intake but reduced body weight gain, adiposity, and microvessel density in adipose tissue, which coincided with reduced expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2. Curcumin increased 5'AMP-activated protein kinase phosphorylation, reduced glycerol-3-phosphate acyl transferase-1, and increased carnitine palmitoyltransferase-1 expression, which led to increased oxidation and decreased fatty acid esterification. The in vivo effect of curcumin on the expression of these enzymes was also confirmed by real-time RT-PCR in subcutaneous adipose tissue. In addition, curcumin significantly lowered serum cholesterol and expression of PPARgamma and CCAAT/enhancer binding protein alpha, 2 key transcription factors in adipogenesis and lipogenesis. The curcumin suppression of angiogenesis in adipose tissue together with its effect on lipid metabolism in adipocytes may contribute to lower body fat and body weight gain. Our findings suggest that dietary curcumin may have a potential benefit in preventing obesity.
Authors:
Asma Ejaz; Dayong Wu; Paul Kwan; Mohsen Meydani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-03-18
Journal Detail:
Title:  The Journal of nutrition     Volume:  139     ISSN:  1541-6100     ISO Abbreviation:  J. Nutr.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-17     Completed Date:  2009-05-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  919-25     Citation Subset:  IM    
Affiliation:
Vascular Biology Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3-L1 Cells
Acetyl-CoA Carboxylase / metabolism
Adenylate Kinase / metabolism
Adipocytes / drug effects*
Adipogenesis / drug effects*
Adipokines / pharmacology
Adipose Tissue / blood supply,  growth & development
Animals
Apoptosis / drug effects
Cell Differentiation / drug effects
Curcumin / administration & dosage*
Diet
Dietary Fats / administration & dosage
Endothelial Cells
Humans
Male
Mice
Mice, Inbred C57BL
Neovascularization, Physiologic / drug effects*
Obesity / prevention & control*
Polymerase Chain Reaction
RNA, Messenger / analysis
Umbilical Veins
Vascular Endothelial Growth Factor A / genetics
Chemical
Reg. No./Substance:
0/Adipokines; 0/Dietary Fats; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 458-37-7/Curcumin; EC 2.7.4.3/Adenylate Kinase; EC 6.4.1.2/Acetyl-CoA Carboxylase

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