Document Detail

Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass.
MedLine Citation:
PMID:  18930198     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the role of the cumulus cells and the cumulus matrix in affecting the penetrability, morphology, acrosome reaction, and motility of human spermatozoa penetrating the cumulus oophorus. DESIGN: Controlled experimental laboratory study. SETTING: University gynecology unit. PATIENT(S): Women undergoing assisted reproduction treatment and men visiting the subfertility clinics. INTERVENTION(S): Human spermatozoa were allowed to penetrate through the cumulus oophorus and cell-depleted cumulus matrix in a capillary, and were treated with cumulus cell extract or hyaluronic acid. MAIN OUTCOME MEASURE(S): The morphology, acrosomal status, and motility of human spermatozoa were determined. RESULT(S): Fewer spermatozoa could penetrate the fresh cell-depleted matrix compared with intact cumulus oophorus. Spermatozoa that penetrated through the cumulus oophorus had higher percentages of normal morphology and acrosome reaction and had specific motility pattern. These effects were lost or reduced in the cell-depleted matrix that had been stored overnight. Hyaluronic acid, a main component of the cumulus matrix at concentration found in the cumulus oophorus, modulated sperm motility but did not affect spontaneous acrosome reaction. Cumulus cell extract did not affect sperm motility, but induced acrosome reaction. CONCLUSION(S): Both the cumulus matrix and the cumulus cells contribute to the effect of cumulus oophorus on spermatozoa penetrating through it.
Shun-Jia Hong; Philip Chi-Ngong Chiu; Kai-Fai Lee; Jenny Yin-Mei Tse; Pak-Chung Ho; William Shu-Biu Yeung
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-18
Journal Detail:
Title:  Fertility and sterility     Volume:  92     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  971-8     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Sun Yat-sen University, Guangdong, China.
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MeSH Terms
Acrosome / drug effects,  physiology
Acrosome Reaction / drug effects,  physiology*
Cell Extracts / pharmacology
Cell Survival / drug effects,  physiology
Cumulus Cells / physiology*
Dose-Response Relationship, Drug
Extracellular Matrix / physiology*
Hyaluronic Acid / pharmacology
Sperm Capacitation / drug effects,  physiology
Sperm Motility / drug effects,  physiology
Sperm-Ovum Interactions / drug effects,  physiology*
Spermatozoa / drug effects,  physiology*
Reg. No./Substance:
0/Cell Extracts; 9004-61-9/Hyaluronic Acid

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