Document Detail

Cumulus cell apoptosis changes with exposure to spermatozoa and pathologies involved in infertility.
MedLine Citation:
PMID:  18692783     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether the incidence of apoptosis in mature oocyte cumulus cells changes after insemination related to infertility. DESIGN: Prospective study. SETTING: Public hospital and university. PATIENT(S): One hundred women undergoing in vitro fertilization and embryo transfer (IVF-ET). INTERVENTION(S): Collection of cumulus cells from IVF-ET cycles with different infertility etiologies. MAIN OUTCOME MEASURE(S): Detection of apoptosis in cumulus cells; fertilization, embryo quality, and pregnancy rate. RESULT(S): The incubation of cumulus-oocyte-complexes with spermatozoa led to an increase in cumulus cell apoptosis from 34.2 +/- 3.7 to 44.5 +/- 6.3%. After insemination, cumulus cells of poor quality embryos showed a statistically higher apoptotic rate versus cumulus cells of good quality embryos (61.5 +/- 6.4 vs. 40.6 +/- 3.9%). Cumulus cells arising from oocytes with >or=50% fertilization rates after insemination showed higher apoptosis rates did cumulus cells from oocytes with <50% fertilization rates (46.0 +/- 3.7 vs. 33.8 +/- 4.0%). Patients with endometriosis presented higher apoptotic rates before insemination (77.6 +/- 9.06%). Cumulus cells obtained after aspiration showed no differences in their apoptosis rates for the following factors: age of women, aspirated oocytes, estradiol level, fertilization rate, and embryo quality or pregnancy. The apoptotic profile from pregnant women was less than (but not statistically significantly different from) profiles from nonpregnant women. CONCLUSION(S): These results suggest that the incidence of apoptosis in cumulus cells is associated with exposure to spermatozoa and the cause of infertility.
Marina Díaz-Fontdevila; Ricardo Pommer; Rosita Smith
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-09
Journal Detail:
Title:  Fertility and sterility     Volume:  91     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2061-8     Citation Subset:  IM    
Institute of Maternal and Child Research, School of Medicine, University of Chile, San Borja Arriarán Clinical Hospital, Santiago, Chile.
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MeSH Terms
Apoptosis / physiology*
Cumulus Cells / cytology*,  physiology
Embryo Transfer / methods
Endometriosis / pathology
Infertility, Female / pathology,  physiopathology
Oocytes / physiology*
Sperm Injections, Intracytoplasmic / methods
Sperm Motility
Spermatozoa / physiology*

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