Document Detail

Cumulative pregnancy rate after ICSI with cryopreserved testicular tissue in non-obstructive azoospermia.
MedLine Citation:
PMID:  15901452     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to describe a simplified and inexpensive method of testicular tissue freezing, to assess the cumulative clinical pregnancy rate (CPR) by this technique, and to provide useful information for counselling couples with non-obstructive azoospermia. One hundred and sixty-five couples with non-obstructive azoospermic males pursuing assisted conception, from December 1995 to December 2002, were included. In all cases, the testicular tissue retrieved by open multiple-biopsy (both sides, by testicular sperm extraction) was frozen using a simple liquid nitrogen vapour freezing technique and was stored in liquid nitrogen thereafter. Only mature spermatozoa were used for intracytoplasmic sperm injection (ICSI) after thawing. Expected CPR were calculated using the Kaplan-Meier survival analysis. A total of 281 cycles were performed resulting in 53 clinical pregnancies. Crude and expected CPR (95% confidence intervals) after three cycles were 32.1 (25.7-40.1) and 55.7% (37.0-74.4) respectively. In conclusion, this simplified method for freezing testicular tissue resulted in a satisfactory outcome after ICSI in cases of non-obstructive azoospermia.
Konstantinos Dafopoulos; Georg Griesinger; Askan Schultze-Mosgau; Yasser Orief; Beate Schöpper; Nikos Nikolettos; Klaus Diedrich; Safaa Al-Hasani
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  10     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-19     Completed Date:  2005-06-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  461-6     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, University of Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
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MeSH Terms
Cryopreservation* / instrumentation
Equipment Design
Pregnancy Rate*
Sperm Injections, Intracytoplasmic*
Survival Analysis

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