| Culturing of retinal pigment epithelium cells. | |
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MedLine Citation:
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PMID: 19494642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The retinal pigment epithelium (RPE) is a monolayer of cells adjacent to the photoreceptors of the retina. It plays a crucial role in maintaining photoreceptor health and survival. Degeneration or dysfunction of the RPE can lead to photoreceptor degeneration and as a consequence to visual impairment. The most common diseased state of the RPE becomes manifest in age-related macular degeneration, an increasing cause of blindness in the elderly. RPE cells are therefore of great interest to researchers working in the field of tissue engineering and cell transplantation. In fact, studies in animal models have proven that the transplantation of RPE cells can delay the course of photoreceptor degenerative diseases. Although first attempts to transplant RPE cells into the subretinal space in human individuals suffering from age-related macular degeneration were less successful, RPE cell transplantation is still favored as a future therapeutic option, and much work is done to develop and design cell transplants. Cell banking is a prerequisite to have well-differentiated and characterized cells at hand when needed for research purposes, but also for therapeutic approaches. In this chapter the authors will describe methods to isolate, culture and preserve adult human RPE cells for the purpose of RPE cell banking. |
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Authors:
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Monika Valtink; Katrin Engelmann |
Publication Detail:
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Type: Journal Article; Review Date: 2009-06-03 |
Journal Detail:
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Title: Developments in ophthalmology Volume: 43 ISSN: 0250-3751 ISO Abbreviation: Dev Ophthalmol Publication Date: 2009 |
Date Detail:
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Created Date: 2009-06-04 Completed Date: 2009-08-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8010321 Medline TA: Dev Ophthalmol Country: Switzerland |
Other Details:
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Languages: eng Pagination: 109-19 Citation Subset: IM |
Copyright Information:
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Copyright 2009 S. Karger AG, Basel. |
Affiliation:
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Institute of Anatomy, Medical Faculty 'Carl Gustav Caris', TU Dresden, Dresden, Germany. monika.valtink@tu-dresden.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques* Cell Separation / methods* Cryopreservation / methods Culture Media Eye Banks Humans Retinal Pigment Epithelium / cytology* Tissue Scaffolds |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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