| Culturing of human hepatocellular carcinoma. A simple and reproducible method. | |
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MedLine Citation:
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PMID: 2578738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eight permanent human hepatocellular carcinoma (HHC) cell lines were established from 8 individual patients by the use of aspirated needle biopsy specimens (smaller than 0.1 ml in size). The cells grew in clustered form and retained intercellular junctions and canaliculi resembling bile canaliculi. The presence of secreted human alpha-fetoprotein and human albumin was detected in the cultured medium. Hepatitis B surface (HBs) antigen was not found on these cells. Implantation of the cells into athymic mice was followed by the growth of hepatocellular carcinomas and the appearance of human alpha-fetoprotein in the mouse serum. Chromosome analysis of three of the cell lines showed hyperdiploidy in two of them and hypotetraploidy in the other. Enzyme analyses of culture medium and cell homogenates have detected some enzymes characteristic of liver tissue such as gamma-glutamyl transferase, sorbital dehydrogenase, alkaline phosphatase, glutamate dehydrogenase, as well as aspartate and alanine transaminase. These tumor cells have been continuously maintained in culture for over 6 years with no significant changes observed. |
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Authors:
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K Laohathai; N Bhamarapravati |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of pathology Volume: 118 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 1985 Feb |
Date Detail:
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Created Date: 1985-03-11 Completed Date: 1985-03-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 203-8 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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analysis Animals Carcinoma, Hepatocellular / enzymology, genetics, immunology, pathology*, ultrastructure Cell Line Hepatitis B Surface Antigens / analysis Humans Karyotyping Liver Neoplasms / enzymology, genetics, immunology, pathology*, ultrastructure Mice Mice, Nude Neoplasm Transplantation Phenotype Ploidies Transplantation, Heterologous alpha-Fetoproteins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Hepatitis B Surface Antigens; 0/alpha-Fetoproteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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