| Culturing the epiblast cells of the pig blastocyst. | |
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MedLine Citation:
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PMID: 8354665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pig epiblast cells that had been separated from other early embryonic cells were cultured in vitro. A three-step dissection protocol was used to isolate the epiblast from trophectoderm and primitive endoderm before culturing. Blastocysts collected at 7 to 8 days postestrus were immunodissected to obtain the inner cell mass (ICM) and destroy trophectodermal cells. The ICM was cultured for 2 to 3 days on STO feeder cells. The epiblast was then physically dissected free of associated primitive endoderm. Epiblast-derived cells, grown on STO feeders, produced colonies of small cells resembling mouse embryonic stem cells. This primary cell morphology changed as the colonies grew and evolved into three distinct colony types (endodermlike, neural rosette, or complex). Cell cultures derived from these three colony types spontaneously differentiated into numerous specialized cell types in STO co-culture. These included fibroblasts, endodermlike cells, neuronlike cells, pigmented cells, adipogenic cells, contracting muscle cells, dome-forming epithelium, ciliated epithelium, tubule-forming epithelium, and a round amoeboid cell type resembling a plasmacyte after Wright staining. The neuronlike cells, contracting muscle cells, and tubule-forming epithelium had normal karyotypes and displayed finite or undefined life spans upon long-term STO co-culture. The dome-forming epithelium had an indefinite life span in STO co-culture and also retained a normal karyotype. These results demonstrate the in vitro pluripotency of pig epiblast cells and indicate the epiblast can be a source for deriving various specialized cell cultures or cell lines. |
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Authors:
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N C Talbot; C E Rexroad; V G Pursel; A M Powell; N D Nel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 29A ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 1993 Jul |
Date Detail:
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Created Date: 1993-09-17 Completed Date: 1993-09-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 543-54 Citation Subset: IM |
Affiliation:
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U.S. Department of Agriculture, Beltsville Agricultural Research Center, Maryland 20705. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology*, ultrastructure Cells, Cultured Chromosomes / ultrastructure Ectoderm / cytology, ultrastructure Endoderm / cytology, ultrastructure Epithelial Cells Epithelium / ultrastructure Fibroblasts / cytology, ultrastructure Karyotyping Macrophages / cytology, ultrastructure Swine / embryology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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