Document Detail


Culturing the epiblast cells of the pig blastocyst.
MedLine Citation:
PMID:  8354665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pig epiblast cells that had been separated from other early embryonic cells were cultured in vitro. A three-step dissection protocol was used to isolate the epiblast from trophectoderm and primitive endoderm before culturing. Blastocysts collected at 7 to 8 days postestrus were immunodissected to obtain the inner cell mass (ICM) and destroy trophectodermal cells. The ICM was cultured for 2 to 3 days on STO feeder cells. The epiblast was then physically dissected free of associated primitive endoderm. Epiblast-derived cells, grown on STO feeders, produced colonies of small cells resembling mouse embryonic stem cells. This primary cell morphology changed as the colonies grew and evolved into three distinct colony types (endodermlike, neural rosette, or complex). Cell cultures derived from these three colony types spontaneously differentiated into numerous specialized cell types in STO co-culture. These included fibroblasts, endodermlike cells, neuronlike cells, pigmented cells, adipogenic cells, contracting muscle cells, dome-forming epithelium, ciliated epithelium, tubule-forming epithelium, and a round amoeboid cell type resembling a plasmacyte after Wright staining. The neuronlike cells, contracting muscle cells, and tubule-forming epithelium had normal karyotypes and displayed finite or undefined life spans upon long-term STO co-culture. The dome-forming epithelium had an indefinite life span in STO co-culture and also retained a normal karyotype. These results demonstrate the in vitro pluripotency of pig epiblast cells and indicate the epiblast can be a source for deriving various specialized cell cultures or cell lines.
Authors:
N C Talbot; C E Rexroad; V G Pursel; A M Powell; N D Nel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  29A     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-09-17     Completed Date:  1993-09-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  543-54     Citation Subset:  IM    
Affiliation:
U.S. Department of Agriculture, Beltsville Agricultural Research Center, Maryland 20705.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / cytology*,  ultrastructure
Cells, Cultured
Chromosomes / ultrastructure
Ectoderm / cytology,  ultrastructure
Endoderm / cytology,  ultrastructure
Epithelial Cells
Epithelium / ultrastructure
Fibroblasts / cytology,  ultrastructure
Karyotyping
Macrophages / cytology,  ultrastructure
Swine / embryology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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