Document Detail


Culturing, Media, and Handling of Legionella.
MedLine Citation:
PMID:  23150393     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This chapter describes methods for culturing Legionella pneumophila in both complex and defined media. The first protocol describes the use of buffered charcoal yeast extract (BCYE) agar, the solid medium that is most commonly used for culturing L. pneumophila. The next procedure details the cultivation of L. pneumophila in buffered yeast extract (BYE) broth, i.e., the liquid medium version of BCYE agar. We describe how culturing in BYE broth can also be used for investigating proteins that are secreted by the type II secretion system of L. pneumophila. The next part of the chapter explains the cultivation of L. pneumophila in a chemically defined liquid media (CDM). CDM contains a mixture of amino acids, metals, α-ketoglutarate, and pyruvate. Because of its defined nature, CDM provides a simple means for controlling the concentration of nutrients and thereby allows for investigations of physiology and metabolism. To illustrate this point, the use of deferrated CDM for the purpose of assessing Legionella siderophore production is outlined. Finally, the chapter ends with a brief discussion of the storage and shipping of L. pneumophila.
Authors:
Christa H Chatfield; Nicholas P Cianciotto
Related Documents :
24225673 - A comparison of anther and microspore culture as a breeding tool in brassica napus.
24194023 - Embryogenesis and plant regeneration of pakchoi (brassica rapa l. ssp. chinensis) via i...
23466703 - The central role of selenium in the biochemistry and ecology of the harmful pelagophyte...
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  151-162     Citation Subset:  -    
Affiliation:
Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Typing Methods for Legionella.
Next Document:  The Intracellular Metabolism of Legionella by Isotopologue Profiling.