| Culturing the Antarctic nematode Plectus murrayi. | |
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MedLine Citation:
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PMID: 21041395 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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INTRODUCTION The Antarctic terrestrial nematode Plectus murrayi is an excellent model organism for the study of stress response mechanisms in various types of environmental conditions. In this procedure, we describe a method for culturing P. murrayi extracted from soil and sediment samples from the McMurdo (MCM) Dry Valleys of Antarctica. Nematodes are cultured on sand agar plates and incubated at various temperatures, and feed on bacteria growing on the agar media. They can be subcultured and stored at 15°C for >2 mo. This method is easy to carry out and can produce nematodes in quantities sufficient for ecological and molecular studies. |
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Authors:
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Bishwo N Adhikari; Cecilia M Tomasel; Grace Li; Diana H Wall; Byron J Adams |
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Publication Detail:
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Type: Journal Article Date: 2010-11-01 |
Journal Detail:
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Title: Cold Spring Harbor protocols Volume: 2010 ISSN: 1559-6095 ISO Abbreviation: Cold Spring Harb Protoc Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101524530 Medline TA: Cold Spring Harb Protoc Country: United States |
Other Details:
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Languages: eng Pagination: pdb.prot5522 Citation Subset: IM |
Affiliation:
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Department of Biology and Evolutionary Ecology Laboratories, Brigham Young University, Provo, UT 84602, USA. adhikaribn@hotmail.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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