Document Detail


Cultured tumor cells of murine submandibular gland origin: a model to investigate pHi regulation of salivary cells.
MedLine Citation:
PMID:  10706478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracellular pH (pHi) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17+/-0.07. To investigate the presence of Na+/H+ and Cl-/HCO3- antiports as well as Na+/K+/2Cl- symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity to regulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells.
Authors:
E Trzaskawka; J Vigo; J C Egea; M C Goldsmith; J M Salmon; D D De Periere
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of oral sciences     Volume:  108     ISSN:  0909-8836     ISO Abbreviation:  Eur. J. Oral Sci.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-09     Completed Date:  2000-03-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9504563     Medline TA:  Eur J Oral Sci     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  54-8     Citation Subset:  D; IM    
Affiliation:
Faculty of Odontology, Laboratory of Physiology, University of Montpellier I, France. trzaskaw@univ-perp.fr
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MeSH Terms
Descriptor/Qualifier:
Acid-Base Equilibrium
Ammonium Chloride / diagnostic use
Animals
Antiporters / antagonists & inhibitors,  metabolism
Biological Transport, Active
Carrier Proteins / antagonists & inhibitors,  metabolism
Chloride-Bicarbonate Antiporters
Fluorometry
Hydrogen / metabolism*
Hydrogen-Ion Concentration
Intracellular Membranes / metabolism
Ion Transport*
Mice
Microscopy, Video
Models, Biological
Sodium-Hydrogen Antiporter / antagonists & inhibitors,  metabolism
Sodium-Potassium-Chloride Symporters
Submandibular Gland / cytology*,  metabolism*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antiporters; 0/Carrier Proteins; 0/Chloride-Bicarbonate Antiporters; 0/Sodium-Hydrogen Antiporter; 0/Sodium-Potassium-Chloride Symporters; 12125-02-9/Ammonium Chloride; 1333-74-0/Hydrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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