Document Detail


Cultured ruminal epithelial cells express a large-conductance channel permeable to chloride, bicarbonate, and acetate.
MedLine Citation:
PMID:  18716793     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The absorption of short-chain fatty acids (SCFA) from the rumen requires efficient mechanisms for both apical uptake and basolateral extrusion. Previous studies suggest that the rumen expresses a basolateral chloride conductance that might be permeable to SCFA. In order to characterize this conductance in more detail, isolated cultured ruminal epithelial cells were studied with the patch-clamp technique, revealing a whole-cell conductance with p(Cl(-)) approximately p(NO(3) (-)) > p(HCO(3) (-)) > p(acetate(-)) > p(gluconate(-)). Currents could be blocked by diisothiocyanato-stilbene-2,2'-disulfonic acid (1 mmol l(-1) > 100 micromol l(-1)), 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (50 micromol l(-1)), niflumic acid (100 micromol l(-1)), and p-chloromercuribenzoate (1 mmol l(-1)). Single-channel conductance was 350 +/- 7 pS for chloride and 142 +/- 7 pS for acetate. Open probability could be fitted with a three-state gating model. We propose a role for this channel in mediating the permeation of chloride, bicarbonate, and acetate across the basolateral membrane of the ruminal epithelium.
Authors:
Friederike Stumpff; Holger Martens; Sabine Bilk; Jörg R Aschenbach; Gotthold Gäbel
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Publication Detail:
Type:  Journal Article     Date:  2008-08-21
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  457     ISSN:  1432-2013     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1003-22     Citation Subset:  IM    
Affiliation:
Institut für Veterinär-Physiologie, Freie Universität Berlin, Germany. stumpff@zedat.fu-berlin.de
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Acetates / metabolism*
Animals
Bicarbonates / metabolism*
Cells, Cultured
Chlorides / metabolism*
Epithelial Cells / metabolism
Fatty Acids, Volatile / metabolism
Gluconates / metabolism
Ion Channel Gating / drug effects*
Nitrates / metabolism
Nitrobenzoates / pharmacology
Patch-Clamp Techniques
Potassium / metabolism
Rumen / cytology,  metabolism*
Sheep
Sodium / metabolism
p-Chloromercuribenzoic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Acetates; 0/Bicarbonates; 0/Chlorides; 0/Fatty Acids, Volatile; 0/Gluconates; 0/Nitrates; 0/Nitrobenzoates; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 526-95-4/gluconic acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 59-85-8/p-Chloromercuribenzoic Acid; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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