Document Detail

Cultured human monocytes secrete fibronectin in response to activation by proinflammatory cytokines.
MedLine Citation:
PMID:  10759765     Owner:  NLM     Status:  MEDLINE    
We studied the effects of the cytokines IL-1alpha, IL-6, tumour necrosis factor-alpha (TNF-alpha), IL-4, IL-10, IL-13 and transforming growth factor-beta (TGF-beta) on fibronectin (FN) production by cultured-human monocytes. IL-1alpha, IL-6 and TNF-alpha all increased FN production, an indicator of monocyte activation. These cytokines increased FN production in a dose-dependent fashion, with a 4-h treatment being sufficient to measure FN production by radioimmunoassay. Conversely, IL-4, IL-10 and IL-13 strongly inhibited cytokine-induced FN production, while TGF-beta only partially inhibited FN production. The combination of suboptimal doses of cytokines (IL-1alpha + IL-6, IL-1alpha + TNF-alpha, IL-6 + TNF-alpha), which could not singly induce substantial amounts of FN, were able to induce FN production by cultured monocytes. Northern blot analysis with a cDNA specific for FN confirmed the expression of FN mRNA in cultured monocytes stimulated with a single cytokine or a combination of cytokines. Our data demonstrate that monocytes may not always require high concentrations of cytokines for activation in vitro, and that the synergistic or additive action of low levels of cytokines on monocyte activation may be sufficient to promote immune or inflammatory reactions. Our data also suggest that certain T cell cytokines may regulate monocyte activation.
N Kitamura; S Nishinarita; T Takizawa; Y Tomita; T Horie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  120     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-08     Completed Date:  2000-05-08     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  66-70     Citation Subset:  IM    
First Department of Internal Medicine, Nihon University School of Medicine, Itabashi, Tokyo, Japan.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Cells, Cultured
Cytokines / physiology*
Dose-Response Relationship, Immunologic
Fibronectins / biosynthesis,  genetics,  secretion*
Inflammation / immunology
Interleukin-1 / physiology
Interleukin-6 / pharmacology
Monocytes / metabolism,  secretion*
RNA, Messenger / biosynthesis
Tumor Necrosis Factor-alpha / pharmacology
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Cytokines; 0/Fibronectins; 0/Interleukin-1; 0/Interleukin-6; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha

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