| Cultured epithelioid astrocytes migrate after transplantation into the adult rat brain. | |
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MedLine Citation:
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PMID: 1955586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A highly purified population of dividing epithelioid astrocytes has been prepared from postnatal rat corpus callosum. These cells were labelled in culture by incorporation of either [3H]thymidine or fluorescent microspheres and transplanted in a fibrin clot into the hippocampi of adult syngeneic rats. Transplanted cells divided in vivo and progressively migrated into the host brain from the site of implantation up to distances of about 1 mm. After a 1-week survival, transplant cells stained strongly for glial fibrillary acidic protein and had the thick sinuous processes characteristic of stellate astrocytes. Artefactual transfer of radiolabel to host cells was ruled out by control experiments in which either the proportion of transplant cells that were radiolabelled was varied or radiolabelled transplant cells were killed prior to implantation. Astrocyte migration over the first days after implantation was determined to occur at a rate of approximately 100 microns per day. Transplant cells moved into both grey and white matter areas of the host brain and over the migratory period were commonly observed to be associated with blood vessels. Some transplant cells were directly juxtaposed against neuronal perikarya and dendrites. Many labelled astrocytes were located in areas that were apparently completely free of damage caused by implantation. These results define a class of mature astrocytic cells that have the ability to migrate through the adult brain. The existence of pathways for cell movement in the adult CNS has implications for the mechanisms of tissue remodelling after injury and transplantation, for regenerative repair of the CNS, and for the dynamics of cell-cell contacts in the normal adult mammalian brain. |
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Authors:
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C J Emmett; J M Lawrence; G Raisman; P J Seeley |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 311 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1991-12-30 Completed Date: 1991-12-30 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 330-41 Citation Subset: IM |
Affiliation:
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Norman and Sadie Lee Research Centre, National Institute for Medical Research, London, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology, physiology, transplantation* Autoradiography Brain Tissue Transplantation / physiology* Cell Movement Corpus Callosum / physiology, transplantation* Epithelial Cells Epithelium / physiology, transplantation Hippocampus / cytology, physiology Rats Rats, Inbred Strains Thymidine / metabolism Transplantation, Heterotopic Transplantation, Isogeneic Tritium |
| Chemical | |
Reg. No./Substance:
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10028-17-8/Tritium; 50-89-5/Thymidine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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