| Cultured epidermal autografts and allodermis combination for permanent burn wound coverage. | |
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MedLine Citation:
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PMID: 8198745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultured epidermal autografts (CEA) have been shown to be an effective permanent skin replacement for major burn injuries, but are more sensitive to adverse conditions than split thickness grafts (Clarke et al., 1988). Cuono et al. (1986, 1987) have described the successful use of engrafted allodermis as a wound bed for cultured grafts. We report on a method of preparing allodermis and grafting CEA in five patients with major burns (48-70 per cent TBSA, average 59.6 per cent). The average age was 38.8 years (20-60 years). All full thickness wounds were excised down to fat within 7 days of admission, and covered with meshed split thickness cryopreserved homograft. Over the ensuing 2-3 weeks, the homograft became engrafted. At surgery, the allo-epidermis was removed, leaving the dermal components as a viable bed for the CEA. Keratinocytes derived from a full thickness biopsy were grown to confluence by the method of Rheinwald and Green (1975), and 25 cm2 sheets were stapled to Vaseline gauze backings and applied to freshly excised wounds. Seven to 10 days after surgery, the gauze backings were removed. The average take ranged from 87-100 per cent (average 93.6 per cent). Follow-up for up to 4 years shows supple skin that has been durable, and resistant to trauma and infection. |
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Authors:
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W L Hickerson; C Compton; S Fletchall; L R Smith |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Burns : journal of the International Society for Burn Injuries Volume: 20 Suppl 1 ISSN: 0305-4179 ISO Abbreviation: Burns Publication Date: 1994 |
Date Detail:
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Created Date: 1994-07-07 Completed Date: 1994-07-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8913178 Medline TA: Burns Country: ENGLAND |
Other Details:
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Languages: eng Pagination: S52-5; discussion S55-6 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Tennessee, Memphis. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Burns / surgery* Cells, Cultured Epidermis / cytology, transplantation* Female Humans Keratinocytes / cytology, transplantation Male Middle Aged Skin / pathology Skin Transplantation* / methods Transplantation, Autologous Transplantation, Homologous |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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