| Cultured early goat embryos and cells are susceptible to infection with caprine encephalitis virus. | |
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MedLine Citation:
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PMID: 16859728 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Zona-pellucida-free embryos at 8-16 cell stage were co-cultured for 6 days in an insert over a mixed cell monolayer infected with CAEV-pBSCA. Embryos were washed and transferred to an insert on CAEV indicator goat synovial membrane cells for 6 h, then they were washed and cultivated in B2 Ménézo for 24 h, finally, embryo cells were dissociated and cultivated on a feeder monolayer for 8 days. After 5 weeks, multinucleated giant cells typical of CAEV infection were observed in indicator GSM cell monolayers. In the acellular medium, the early embryonic cells produced at least 10(3.25) TCID50/ml over 24 h. The monolayer of cultivated embryonic cells developed cytopathic lesions within 8 days, and CAEV RNA, CAEV proviral DNA and protein p28 of the capsid were detected. All of these results clearly demonstrate that caprine early embryonic cells are susceptible to infection with CAEV and that infection with this virus is productive. |
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Authors:
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M Z Ali Al Ahmad; F Fieni; F Guiguen; M Larrat; J L Pellerin; C Roux; Y Chebloune |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-21 |
Journal Detail:
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Title: Virology Volume: 353 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2006 Sep |
Date Detail:
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Created Date: 2006-09-25 Completed Date: 2006-11-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 307-15 Citation Subset: IM |
Affiliation:
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Department of Research into the Health Risk and Biotechnology of Reproduction ENVN/DGER, National Veterinary School, BP 40706, 44307 Nantes Cedex 03, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthritis-Encephalitis Virus, Caprine / chemistry, genetics, growth & development* Capsid Proteins / metabolism Cells, Cultured Coculture Techniques DNA, Viral / genetics Embryo, Mammalian / virology Genome, Viral / genetics Goats Immunohistochemistry Lentivirus Infections / virology* Polymerase Chain Reaction Proviruses / genetics, isolation & purification Sensitivity and Specificity Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/Capsid Proteins; 0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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