| Cultured Ehrlich ascites tumor cells show increased N-linked alpha 2,6-sialyltransferase activity. | |
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MedLine Citation:
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PMID: 8512312 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ehrlich ascites tumor cells (EAT cells) are routinely grown in the peritoneal cavity of mice. These cells, EAT-wt, grow in suspension and exhibit a high level of alpha-2,3-O-linked sialyltransferase activity with benzyl-T-antigen (Gal beta 1,3Ga1NAc-alpha-O-CH2C6H5) as acceptor. These cells also contain a very low level of alpha-2,6-O-linked and alpha-2,6-N-linked sialyltransferase activity. A variant of these cells, EAT-c, has been selected to grow in cell culture, attached to the surface of culture flasks. EAT-c cells exhibit a selective increase of two- to fivefold in the activity of alpha-2,6-N-linked sialyltransferase activity, using asialo-alpha 1-acid glycoprotein as acceptor. Since a similar selective increase has been previously observed in metastatic human colorectal cancer tissues, the EAT-wt/EAT-c cell system may serve as a good experimental model for the investigation of sialyltransferases and their cell surface sialylated products in relation to cancer, metastasis, and cell-cell interaction. |
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Authors:
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M K Mostafapour; I J Goldstein |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 303 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-07-09 Completed Date: 1993-07-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 255-9 Citation Subset: IM |
Affiliation:
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Department of Natural Sciences, University of Michigan-Dearborn 48128. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Viral, Tumor / metabolism Benzyl Compounds / metabolism Carbohydrate Conformation Carbohydrate Sequence Carcinoma, Ehrlich Tumor / enzymology* Female Mice Molecular Sequence Data N-Acetylneuraminic Acid Neuraminidase / metabolism Orosomucoid / metabolism Sialic Acids / metabolism Sialyltransferases / metabolism* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA-20424/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Viral, Tumor; 0/Benzyl Compounds; 0/Orosomucoid; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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