Document Detail


Cultured Ehrlich ascites tumor cells show increased N-linked alpha 2,6-sialyltransferase activity.
MedLine Citation:
PMID:  8512312     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ehrlich ascites tumor cells (EAT cells) are routinely grown in the peritoneal cavity of mice. These cells, EAT-wt, grow in suspension and exhibit a high level of alpha-2,3-O-linked sialyltransferase activity with benzyl-T-antigen (Gal beta 1,3Ga1NAc-alpha-O-CH2C6H5) as acceptor. These cells also contain a very low level of alpha-2,6-O-linked and alpha-2,6-N-linked sialyltransferase activity. A variant of these cells, EAT-c, has been selected to grow in cell culture, attached to the surface of culture flasks. EAT-c cells exhibit a selective increase of two- to fivefold in the activity of alpha-2,6-N-linked sialyltransferase activity, using asialo-alpha 1-acid glycoprotein as acceptor. Since a similar selective increase has been previously observed in metastatic human colorectal cancer tissues, the EAT-wt/EAT-c cell system may serve as a good experimental model for the investigation of sialyltransferases and their cell surface sialylated products in relation to cancer, metastasis, and cell-cell interaction.
Authors:
M K Mostafapour; I J Goldstein
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  303     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-09     Completed Date:  1993-07-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-9     Citation Subset:  IM    
Affiliation:
Department of Natural Sciences, University of Michigan-Dearborn 48128.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Viral, Tumor / metabolism
Benzyl Compounds / metabolism
Carbohydrate Conformation
Carbohydrate Sequence
Carcinoma, Ehrlich Tumor / enzymology*
Female
Mice
Molecular Sequence Data
N-Acetylneuraminic Acid
Neuraminidase / metabolism
Orosomucoid / metabolism
Sialic Acids / metabolism
Sialyltransferases / metabolism*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-20424/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Viral, Tumor; 0/Benzyl Compounds; 0/Orosomucoid; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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