| Cultured Chinese hamster cells undergo apoptosis after exposure to cold but nonfreezing temperatures. | |
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MedLine Citation:
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PMID: 2394128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultured Chinese hamster V79 fibroblast cells at the transition from logarithmic to stationary growth have been shown to undergo apoptosis (programmed cell death) after cold shock [B. L. Soloff, W. A. Nagle, A. J. Moss, Jr., K. J. Henle, and J. T. Crawford, Biochem. Biophys. Res. Commun. 145, 876-883 (1987)]. In this report, we show that about 95% of the cell population was susceptible to cold-induced apoptosis, and the amount of cell killing was dependent on the duration of hypothermia. Cells treated for 0-90 min at 0 degrees C exhibited an exponential survival curve with a D0 of 32 min; thus, even short exposures to the cold (e.g., 5 min) produced measurable cell killing. The cold-induced injury was not produced by freezing, because similar results were observed at 6 degrees C, and cell killing was not influenced by the cryoprotective agent dimethyl sulfoxide. Cold-induced apoptosis was inhibited by rewarming at 23 degrees C, compared to 37 degrees C, by inhibitors of macromolecular synthesis, such as cycloheximide, and by 0.8 mM zinc sulfate. The results suggest that apoptosis represents a new manifestation of cell injury after brief exposure to 0-6 degrees C hypothermia. |
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Authors:
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W A Nagle; B L Soloff; A J Moss; K J Henle |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cryobiology Volume: 27 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-10-11 Completed Date: 1990-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 439-51 Citation Subset: IM |
Affiliation:
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Department of Radiology (Nuclear Medicine), University of Arkansas for Medical Sciences, Little Rock. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival* / drug effects Cells, Cultured Cold Temperature* Cricetinae Cricetulus Cycloheximide / pharmacology DNA Damage Dimethyl Sulfoxide / pharmacology Freezing Sulfates / pharmacology Temperature Zinc / pharmacology Zinc Sulfate |
| Chemical | |
Reg. No./Substance:
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0/Sulfates; 66-81-9/Cycloheximide; 67-68-5/Dimethyl Sulfoxide; 7440-66-6/Zinc; 7733-02-0/Zinc Sulfate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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