Document Detail


Culture and spray-drying of Tsukamurella paurometabola C-924: stability of formulated powders.
MedLine Citation:
PMID:  17603774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nematocidal agent, Tsukamurella paurometabola C-924, was cultured in a 300 l bioreactor. Spray-dried formulations of this microorganism were prepared using sucrose. At an outlet temperature 62 degrees C, survival rates between 12 and 85% were reached with sucrose up to 10% (w/w). The stability study of the powders showed that the best storage condition was at 4 degrees C under vacuum. A new method for the calculation of cell death order for bacteria stored at low temperatures was developed. Powders stored under vacuum showed an Arrhenius behavior in relation to cell death kinetics.
Authors:
Armando Hernández; Fréderic Weekers; Jesús Mena; Eulogio Pimentel; Jesús Zamora; Carlos Borroto; Philippe Thonart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-30
Journal Detail:
Title:  Biotechnology letters     Volume:  29     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-09-27     Completed Date:  2008-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1723-8     Citation Subset:  IM    
Affiliation:
Center for Genetic Engineering and Biotechnology, P.O. Box 387, Camaguey, 70100, Cuba. armando.hernandez@cigb.edu.cu
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MeSH Terms
Descriptor/Qualifier:
Actinomycetales / growth & development*,  isolation & purification*
Antinematodal Agents / isolation & purification
Bioreactors
Cell Death
Microbial Viability*
Particulate Matter / isolation & purification
Powders
Sucrose / chemistry
Temperature
Chemical
Reg. No./Substance:
0/Antinematodal Agents; 0/Particulate Matter; 0/Powders; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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