Document Detail

Culture of specimens other than sputum for Mycobacteria.
MedLine Citation:
PMID:  4216599     Owner:  NLM     Status:  MEDLINE    
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rate to 0.1%. Neither acid treatment nor antibiotics appeared to reduce the isolation rate. An effective procedure is to treat all specimens (except cerebrospinal fluid) with acid and culture on two Lowenstein-Jensen slopes (one with pyruvate), a 7H11 selective slope, and in two bottles of selective Kirchner medium.
A Mitchison; V R Aber
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  27     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1974 Nov 
Date Detail:
Created Date:  1975-04-17     Completed Date:  1975-04-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  883-7     Citation Subset:  AIM; IM    
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MeSH Terms
Anti-Bacterial Agents
Bacteriological Techniques / methods
Body Fluids / microbiology*
Cerebrospinal Fluid / microbiology
Culture Media
Mycobacterium tuberculosis / isolation & purification*
Sputum / microbiology
Sulfuric Acids
Suppuration / microbiology
Urine / microbiology
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media; 0/Sulfuric Acids

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