| Culture of postnatal mesencephalic dopamine neurons on an astrocyte monolayer. | |
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MedLine Citation:
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PMID: 18633997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This unit presents a protocol for primary culture of postnatal mesencephalic dopamine neurons grown on an astrocyte monolayer, which can be used to investigate cellular and molecular mechanisms regulating dopamine neuron function. Using this in vitro approach, dopamine neurons survive for an extended period of time and establish functional axon terminals and dendrites that display properties similar to those observed in vivo and in brain slices. An alternate protocol is provided for a microculture system in which astrocytes are grown on a spatially limited surface where single or small groups of dopamine neurons develop. Under such conditions, isolated neurons establish synaptic contacts, or autapses, onto their own somatodendritic compartment, thus facilitating morphological and physiological experiments. |
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Authors:
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Caroline Fasano; Dominic Thibault; Louis-Eric Trudeau |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] Volume: Chapter 3 ISSN: 1934-8576 ISO Abbreviation: Curr Protoc Neurosci Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-17 Completed Date: 2008-10-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706581 Medline TA: Curr Protoc Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: Unit 3.21 Citation Subset: IM |
Copyright Information:
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Copyright 2008 by John Wiley & Sons, Inc. |
Affiliation:
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Département de Pharmacologie, Faculté de Médecine, Université de Montréal, Québec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Astrocytes / cytology*, metabolism Axons / metabolism, ultrastructure Cell Communication / physiology Cell Culture Techniques / methods Cell Differentiation / physiology Cells, Cultured Dendrites / metabolism, ultrastructure Dopamine / metabolism* Mice Neural Pathways / cytology, growth & development Neurons / cytology*, metabolism Rats Substantia Nigra / cytology*, growth & development Synapses / physiology, ultrastructure Ventral Tegmental Area / cytology*, growth & development |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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