Document Detail


Culture methods of allograft musculoskeletal tissue samples in Australian bacteriology laboratories.
MedLine Citation:
PMID:  23314796     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Samples of allograft musculoskeletal tissue are cultured by bacteriology laboratories to determine the presence of bacteria and fungi. In Australia, this testing is performed by 6 TGA-licensed clinical bacteriology laboratories with samples received from 10 tissue banks. Culture methods of swab and tissue samples employ a combination of solid agar and/or broth media to enhance micro-organism growth and maximise recovery. All six Australian laboratories receive Amies transport swabs and, except for one laboratory, a corresponding biopsy sample for testing. Three of the 6 laboratories culture at least one allograft sample directly onto solid agar. Only one laboratory did not use a broth culture for any sample received. An international literature review found that a similar combination of musculoskeletal tissue samples were cultured onto solid agar and/or broth media. Although variations of allograft musculoskeletal tissue samples, culture media and methods are used in Australian and international bacteriology laboratories, validation studies and method evaluations have challenged and supported their use in recovering fungi and aerobic and anaerobic bacteria.
Authors:
Kerry Varettas
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Cell and tissue banking     Volume:  -     ISSN:  1573-6814     ISO Abbreviation:  Cell Tissue Bank     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965121     Medline TA:  Cell Tissue Bank     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
South Eastern Area Laboratory Services, Microbiology Department, CSB Level 3, The St. George Public Hospital, Gray St, Kogarah, Sydney, NSW, 2217, Australia, Kerry.Varettas@sesiahs.health.nsw.gov.au.
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