| Culture of mesothelial cells from bovine pericardium and characterization of their arachidonate metabolism. | |
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MedLine Citation:
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PMID: 3115306 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultures of mesothelial cells from bovine pericardium were established and their arachidonate metabolism was characterized. The identification of the cultured cells was based on morphological observations, and by electrophoretic analysis of cytoskeletal proteins, which demonstrated a pattern previously reported for mesothelial cells. Factor VIII-related antigen was present by indirect immunofluorescence, but the cells had no thrombomodulin activity. The cultured pericardial cells metabolized arachidonic acid to 6-ketoprostaglandin F1 alpha and a small amount of prostaglandin E2. The same metabolites were produced by pieces of intact parietal pericardium but not by pieces from which mesothelium had been removed. The cultured mesothelial cells produced 94.6 +/- 60.4 (mean +/- S.D.) ng/mg (n = 3) cell protein of 6-ketoprostaglandin F1 alpha in response to the calcium ionophore A23187, 117.3 +/- 13.6 ng/mg (n = 3) with exogenous arachidonic acid, 18.3 +/- 11.3 ng/mg (n = 5) with bradykinin, 8.4 +/- 4.3 ng/kg (n = 4) with histamine and 11.2 +/- 9.7 ng/mg (n = 5) with thrombin. All of these values were significantly higher (P less than 0.05) than the control (2.1 +/- 1 ng/mg; n = 5). From these results, we conclude that the mesothelial cells account for the arachidonate metabolism in the pericardium. The production of prostaglandin I2 occurs in response to physiological or pathological, agonists, and is substantial. That is, it is approximately the same as endothelial cells. The release of eicosanoids by mesothelial cells into the pericardial space may have a significant role in cardiac physiology and pathology. |
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Authors:
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K Satoh; S M Prescott |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 930 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1987 Oct |
Date Detail:
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Created Date: 1987-11-09 Completed Date: 1987-11-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 283-96 Citation Subset: IM |
Affiliation:
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Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah School of Medicine, Salt Lake City. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens / analysis Arachidonic Acid Arachidonic Acids / metabolism* Cattle Cells, Cultured Culture Techniques / methods Cytoskeletal Proteins / analysis Factor VIII / analysis, immunology Microscopy, Electron, Scanning Pericardium / cytology*, metabolism, ultrastructure von Willebrand Factor / analysis |
| Grant Support | |
ID/Acronym/Agency:
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HL34127/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Arachidonic Acids; 0/Cytoskeletal Proteins; 0/von Willebrand Factor; 506-32-1/Arachidonic Acid; 9001-27-8/Factor VIII |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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