Document Detail


Culture media for the isolation of Cronobacter spp.
MedLine Citation:
PMID:  19896231     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Enterobacter sakazakii is a member of the Enterobacteriaceae and has been implicated in causing necrotising enterocolitis, as well as bacteraemia and meningitis in infants. In some cases, the infection has been linked to ingestion of infant formula milk (IFM) that has not been terminally sterilised. The nomenclature of E. sakazakii has been clarified and it has now been accepted as a group of six species comprising a novel genus, Cronobacter. Outbreaks in neonatal intensive care units resulting in relatively high case fatality rates and the recognition of IFM as a significant route of infection prompted the development of culture-based detection methods. Development of enrichment broths specific for Cronobacter spp., coupled to the use of fluorogenic and chromogenic substrates in culture media has significantly improved the sensitivity and specificity of methods. This review presents the history and rationale behind the currently available methods, and gives an overview of the principles involved in designing these microbiological media.
Authors:
Patrick Druggan; Carol Iversen
Publication Detail:
Type:  Journal Article     Date:  2009-09-22
Journal Detail:
Title:  International journal of food microbiology     Volume:  136     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  169-78     Citation Subset:  IM    
Affiliation:
Oxoid Ltd., Thermo Fisher Scientific, Basingstoke, Hampshire RG24 8PW, United Kingdom. peter.kuhnert@vbi.unibe.ch
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