Document Detail


Culture of major pelvic ganglion neurons from adult rat.
MedLine Citation:
PMID:  23283520     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology and staining with neuronal marker (neurofilament-200, NF-200). The results demonstrate that the new protocol we used was reliable in obtaining a relatively high yield of MPG neurons. Furthermore, it improves the speed and simplicity in neuronal isolation. The viability of neurons can be maintained for about 2 weeks, which should be sufficient for investigating physiological and pathological processes occurring in mature major pelvic ganglia. And this may provide a useful assessment to currently available techniques for the culture of adult neurons.
Authors:
Shigang Cheng; Xinghai Yang; Yifan Zhang; Chuanguo Xiao
Publication Detail:
Type:  Journal Article     Date:  2013-01-03
Journal Detail:
Title:  Cytotechnology     Volume:  65     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-24     Completed Date:  2013-07-24     Revised Date:  2014-08-01    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  663-9     Citation Subset:  -    
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