Document Detail

Culture-independent analysis of fecal microbiota in infants, with special reference to Bifidobacterium species.
MedLine Citation:
PMID:  15686844     Owner:  NLM     Status:  MEDLINE    
Fecal microbiota of 31 breast-fed, 26 mix-fed, and 11 bottle-fed infants were analyzed by using terminal restriction fragment length polymorphism (T-RFLP), and culture method. We first determined the total and cultivated bacterial counts in infant fecal microbiota. Only approximately 30% of bacteria present in fecal microbiota were cultivable while the remainder was yet-to-be cultured bacteria. Sixty-eight fecal samples were divided into two clusters (I and II) by T-RFLP analysis, and then subdivided into five subclusters (Ia, Ib, IIa, IIb and IIc). There was no clear relationship between clusters and feeding method. A proportion of bifidobacteria was detected in the fecal material by PCR method using species-specific primers. The predominant Bifidobacterium spp. was Bifidobacterium longum longum type (43 samples (63.2%)), followed by B. longum infantis type (23 samples (33.8%)) and B. breve (16 samples (23.5%)). The distribution of Bifidobacterium spp. was similar in the three feeding groups. In contrast, the high incidence of B. breve in cluster I, especially subcluster Ia and B. longum longum type in cluster II, especially subcluster IIa and IIc were characterized by T-RFLP method. Our results showed that the colonization of Bifidobacterium spp. in infant feces correlated with the T-RFLP clusters.
Shinji Sakata; Toshiki Tonooka; Shinobu Ishizeki; Masaaki Takada; Mitsuo Sakamoto; Masafumi Fukuyama; Yoshimi Benno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  243     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-02     Completed Date:  2005-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  417-23     Citation Subset:  IM    
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
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MeSH Terms
Bifidobacterium / classification,  genetics,  isolation & purification*
Bottle Feeding*
Breast Feeding*
Colony Count, Microbial
Culture Media
Feces / microbiology*
Infant, Newborn
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reg. No./Substance:
0/Culture Media

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