Document Detail


Culture of human endometrial cells: a new simple technique to completely separate epithelial glands.
MedLine Citation:
PMID:  7680517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology.
Authors:
P Viganò; A M Di Blasio; G Dell'Antonio; M Vignali
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta obstetricia et gynecologica Scandinavica     Volume:  72     ISSN:  0001-6349     ISO Abbreviation:  Acta Obstet Gynecol Scand     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-04-08     Completed Date:  1993-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370343     Medline TA:  Acta Obstet Gynecol Scand     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  87-92     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, University of Milano, Italy.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation / methods*
Cells, Cultured / cytology*
Endometrium / chemistry,  cytology*
Epithelial Cells
Female
Humans
Immunohistochemistry
Keratins / analysis
Stromal Cells / cytology
Vimentin / analysis
Chemical
Reg. No./Substance:
0/Vimentin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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