| Culture of human endometrial cells: a new simple technique to completely separate epithelial glands. | |
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MedLine Citation:
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PMID: 7680517 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro studies on dispersed human endometrial cells are still difficult to perform as the techniques employed do not allow an optimal separation of the cells. In particular, epithelial glands tend to maintain their tubular structure even after enzymatic dispersion. This could be a disadvantage when monolayers of single well dispersed cells are needed. In this paper we describe a new technique to establish monolayer cultures of isolated endometrial stromal and epithelial cell populations. After a first collagenase digestion, stromal and epithelial cells were separated by differential sedimentation at unity gravity. The epithelial glands obtained were further dispersed in single cells using a short incubation with low amount of trypsin. Morphologic characterization was performed using immunohistochemistry for vimentin and cytokeratins. Compared to previous described methods, this procedure is shorter and could better preserve cell surface structures. Thus, it could be successfully employed for in vitro studies of human endometrial pathophysiology. |
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Authors:
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P Viganò; A M Di Blasio; G Dell'Antonio; M Vignali |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta obstetricia et gynecologica Scandinavica Volume: 72 ISSN: 0001-6349 ISO Abbreviation: Acta Obstet Gynecol Scand Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-04-08 Completed Date: 1993-04-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370343 Medline TA: Acta Obstet Gynecol Scand Country: DENMARK |
Other Details:
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Languages: eng Pagination: 87-92 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, University of Milano, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation
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methods* Cells, Cultured / cytology* Endometrium / chemistry, cytology* Epithelial Cells Female Humans Immunohistochemistry Keratins / analysis Stromal Cells / cytology Vimentin / analysis |
| Chemical | |
Reg. No./Substance:
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0/Vimentin; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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