| Culture & characterisation of limbal epithelial cells & oral mucosal cells. | |
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MedLine Citation:
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PMID: 20418557 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & OBJECTIVES: Sources of autologous tissue that can functionally replace the corneal epithelium have been considered as an alternative to allogenous limbal transplants for limbal stem cells deficiency (LSCD). The aim of the present study was to compare the characterization of oral mucosa with limbal epithelial cells by markers using reverse transcriptase polymerase chain reaction (RT-PCR). METHODS: Experiments were performed using oral tissue (n=6) obtained from patients who underwent oral mucosal graft for LSCD. Confluent cultures of limbus and oral mucosa epithelial cells were characterized by the pututative stem cell markers using RT-PCR. The morphological characteristics of cultivated epithelial cells were analyzed by haematoxylin and eosin staining and phase contrast microscopy. RESULTS: Confluent sheets of epithelial cells were seen at the end of 14(th) day resembling the morphological features of limbal epithelia. RT-PCR analysis showed that cultured oral epithelial cells expressed markers such as ABCG2, p63, delta Np63, isoforms of p63, Keratin 3 (K3), membrane protein--Mucin (MUC 1, 4 and 16) and Antimicrobial Peptide--AMP (Human beta Defensin--hBD 1, 2 and 3). INTERPRETATION & CONCLUSIONS: Oral epithelial cultures have morphological features resembling corneal and limbal epithelial cells by expressing similar marker genes. Thus, feasibility of clinical use of oral epithelial cells need be evaluated for allogenous limbal transplants. |
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Authors:
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Sasirekha Krishnan; Geetha Krishnan Iyer; Subramanian Krishnakumar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Indian journal of medical research Volume: 131 ISSN: 0971-5916 ISO Abbreviation: Indian J. Med. Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-07-29 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 0374701 Medline TA: Indian J Med Res Country: India |
Other Details:
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Languages: eng Pagination: 422-8 Citation Subset: IM |
Affiliation:
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L & T Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, India. drkrishnakumar_2000@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antimicrobial Cationic Peptides
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pharmacology Cells, Cultured Cornea / pathology Corneal Transplantation / methods* Epithelial Cells / cytology* Humans Limbic System / pathology*, transplantation Membrane Proteins / chemistry Microscopy, Phase-Contrast / methods Mouth Mucosa / pathology Mucins / metabolism Protein Isoforms Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology Transplantation / methods* |
| Chemical | |
Reg. No./Substance:
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0/Antimicrobial Cationic Peptides; 0/CKAP4 protein, human; 0/Membrane Proteins; 0/Mucins; 0/Protein Isoforms |
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