Document Detail

Culture & characterisation of limbal epithelial cells & oral mucosal cells.
MedLine Citation:
PMID:  20418557     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & OBJECTIVES: Sources of autologous tissue that can functionally replace the corneal epithelium have been considered as an alternative to allogenous limbal transplants for limbal stem cells deficiency (LSCD). The aim of the present study was to compare the characterization of oral mucosa with limbal epithelial cells by markers using reverse transcriptase polymerase chain reaction (RT-PCR).
METHODS: Experiments were performed using oral tissue (n=6) obtained from patients who underwent oral mucosal graft for LSCD. Confluent cultures of limbus and oral mucosa epithelial cells were characterized by the pututative stem cell markers using RT-PCR. The morphological characteristics of cultivated epithelial cells were analyzed by haematoxylin and eosin staining and phase contrast microscopy.
RESULTS: Confluent sheets of epithelial cells were seen at the end of 14(th) day resembling the morphological features of limbal epithelia. RT-PCR analysis showed that cultured oral epithelial cells expressed markers such as ABCG2, p63, delta Np63, isoforms of p63, Keratin 3 (K3), membrane protein--Mucin (MUC 1, 4 and 16) and Antimicrobial Peptide--AMP (Human beta Defensin--hBD 1, 2 and 3).
INTERPRETATION & CONCLUSIONS: Oral epithelial cultures have morphological features resembling corneal and limbal epithelial cells by expressing similar marker genes. Thus, feasibility of clinical use of oral epithelial cells need be evaluated for allogenous limbal transplants.
Sasirekha Krishnan; Geetha Krishnan Iyer; Subramanian Krishnakumar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Indian journal of medical research     Volume:  131     ISSN:  0971-5916     ISO Abbreviation:  Indian J. Med. Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-29     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0374701     Medline TA:  Indian J Med Res     Country:  India    
Other Details:
Languages:  eng     Pagination:  422-8     Citation Subset:  IM    
L & T Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, India.
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MeSH Terms
Antimicrobial Cationic Peptides / pharmacology
Cells, Cultured
Cornea / pathology
Corneal Transplantation / methods*
Epithelial Cells / cytology*
Limbic System / pathology*,  transplantation
Membrane Proteins / chemistry
Microscopy, Phase-Contrast / methods
Mouth Mucosa / pathology
Mucins / metabolism
Protein Isoforms
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology
Transplantation / methods*
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/CKAP4 protein, human; 0/Membrane Proteins; 0/Mucins; 0/Protein Isoforms

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